Manganese-enhanced magnetic resonance imaging (MEMRI) without compromise of the blood-brain barrier detects hypothalamic neuronal activity in vivo
Manganese-enhanced magnetic resonance imaging (MEMRI) without compromise of the blood-brain barrier detects hypothalamic neuronal activity in vivo
复制标题
DOI：
10.1002/nbm.1070
复制
发表时间：
2006-12-01
影响因子：
2.9
通讯作者：
Bell, Jimmy D.
中科院分区：
文献类型：
--
作者：
Kuo, Yu-Ting;Herlihy, Amy H.;Bell, Jimmy D.
在动物模型中，使用锰增强磁共振成像(MEMRI)来检测神经元的活动和结构越来越引起人们的兴趣。MEMRI神经元活动的研究通常是通过立体定向脑注射或全身注射结合血脑屏障(BBB)的方式进行的。然而，这些方法限制了MEMRI的使用，因为这些方法与手术相关的发病率/死亡率，或者因为这些方法测量的大脑活动可能偏离真实的生理反应。在这项研究中，测试了MEMRI的假设，即在不损害血脑屏障完整性的情况下，全身给予Mn(2+)进行MEMRI能够检测与进食相关的下丘脑功能。这一过程是在一个简单的生理条件下进行的，禁食，用这种方法可以检测到Mn(2+)增强的时间和区域差异。结论：MEMRI可用于研究小鼠脑内下丘脑功能，而不影响血脑屏障。研究还表明，禁食可以调节小鼠脑内区域特异性的Mn(2+)增强。更重要的是，这种非侵入性的体内成像技术能够显示大脑活动的差异，而以前只有通过体外研究才能做到这一点。版权所有(C)2006 John Wiley&Sons，Ltd.
There is growing interest in the use of manganese-enhanced MRI (MEMRI) to detect neuronal activity and architecture in animal models. The MEMRI neuronal activity studies have been generally performed either by stereotactic brain injection or by systemic administration of Mn(2+) in conjunction with the disruption of the blood-brain barrier (BBB). These approaches, however, have limited the use of MEMRI because of the procedure-related morbidity/mortality or because brain activity measured by these methods can diverge from genuine physiological responses. In this study, the hypothesis that MEMRI, performed with systemic administration of Mn(2+) without compromising the BBB integrity, is able to detect hypothalamic function associated with feeding was tested. This procedure was tested on a simple physiological condition, fasting, and with this method temporal and regional differences in Mn(2+) enhancement could be detected. It is concluded that MEMRI can be used to study hypothalamic function in the murine brain without compromising the BBB. It was also shown that region-specific Mn(2+) enhancement in the mouse brain can be modulated by fasting. More importantly, this non-invasive in vivo imaging technique is able to demonstrate differences in brain activities, previously possible only by in vitro studies. Copyright (C) 2006 John Wiley & Sons, Ltd.