α- and β-Adrenergic pathways differentially regulate cell type-specific apoptosis in rat cardiac myocytes
α- and β-Adrenergic pathways differentially regulate cell type-specific apoptosis in rat cardiac myocytes
复制标题
DOI：
10.1161/01.cir.100.3.305
复制
发表时间：
1999-07-20
期刊：
影响因子：
37.8
通讯作者：
Sasayama, S
中科院分区：
文献类型：
--
作者：
Iwai-Kanai, E;Hasegawa, K;Sasayama, S
背景：心肌细胞凋亡可能在心力衰竭的发生发展中起重要作用。去甲肾上腺素是心力衰竭的激活因子之一，在培养中可诱导心肌细胞凋亡。然而，尚不清楚α -和β -肾上腺素能通路是否协调或差异调节细胞凋亡，以及这种凋亡通路是否使用共同的或细胞类型特异性的凋亡信号。方法和结果：我们用α(1)-肾上腺素激动剂（PE, phenylephrine）、β -肾上腺素激动剂（异丙肾上腺素[Iso]）或膜渗透性cAMP类似物（8-Br-cAMP）在无血清条件下刺激培养的新生大鼠心肌细胞48小时。与生理盐水刺激相比，Iso和8-Br-cAMP显著增加了tunel阳性细胞的数量（% tunel阳性细胞核>40%）(
Background-The apoptosis of cardiac myocytes may play a role in the development of heart failure. Norepinephrine is one of the factors activated in heart failure and can induce myocardial cell apoptosis in culture. However, it is unknown if alpha- and beta-adrenergic pathways coordinately or differentially regulate apoptosis and if this apoptotic pathway uses common or cell type-specific apoptotic signals.Methods and Results-We stimulated cultured neonatal rat cardiac myocytes with an alpha(1)-adrenergic agonist (PE, phenylephrine), a beta-adrenergic agonist (isoproterenol [Iso]) or a membrane-permeable cAMP analogue (8-Br-cAMP) in serum-free conditions for 48 hours. Iso and 8-Br-cAMP markedly increased the number of TUNEL-positive cells (%TUNEL-positive nuclei >40%) compared with saline stimulation (