Rapidly-Customizable, Scalable 3D-Printed Wireless Optogenetic Probes for Versatile Applications in Neuroscience
Rapidly-Customizable, Scalable 3D-Printed Wireless Optogenetic Probes for Versatile Applications in Neuroscience
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DOI：
10.1002/adfm.202004285
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发表时间：
2020-09-18
影响因子：
19
通讯作者：
Jeong, Jae-Woong
中科院分区：
文献类型：
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作者：
Lee, Juhyun;Parker, Kyle E.;Jeong, Jae-Woong
光遗传学是一种先进的神经科学技术，它能够以高时空精度解剖神经电路。材料和微制造技术的最新进展使微创和生物兼容的光学神经探针成为可能，从而促进了体内光遗传学研究。然而，传统的制造技术依赖于洁净室设施，这些设施不容易进入，使用起来也很昂贵，使得整个制造过程不便且成本高昂。此外，当前制造程序固有的耗时性质阻碍了在活体研究之间快速定制神经探针。在这里，介绍了一种源于3D打印技术的新技术，用于低成本、大规模生产快速定制的光遗传神经探针。详细介绍了3D打印的生产过程、即时设计的多功能性、3D打印的光遗传探针的生物兼容性以及它们在体内无线光遗传学中的功能能力。3D打印设备的成功活体研究突显了这种易于获取和灵活的制造方法的可靠性，随着打印技术的进步，它可以预示着它在未来低成本生物电子产品中的广泛应用。
Optogenetics is an advanced neuroscience technique that enables the dissection of neural circuitry with high spatiotemporal precision. Recent advances in materials and microfabrication techniques have enabled minimally invasive and biocompatible optical neural probes, thereby facilitating in vivo optogenetic research. However, conventional fabrication techniques rely on cleanroom facilities, which are not easily accessible and are expensive to use, making the overall manufacturing process inconvenient and costly. Moreover, the inherent time-consuming nature of current fabrication procedures impede the rapid customization of neural probes in between in vivo studies. Here, a new technique stemming from 3D printing technology for the low-cost, mass production of rapidly customizable optogenetic neural probes is introduced. The 3D printing production process, on-the-fly design versatility, and biocompatibility of 3D printed optogenetic probes as well as their functional capabilities for wireless in vivo optogenetics is detailed. Successful in vivo studies with 3D printed devices highlight the reliability of this easily accessible and flexible manufacturing approach that, with advances in printing technology, can foreshadow its widespread applications in low-cost bioelectronics in the future.