Expression profiling analysis of long noncoding RNAs in a mouse model of ventilator-induced lung injury indicating potential roles in inflammation.
Expression profiling analysis of long noncoding RNAs in a mouse model of ventilator-induced lung injury indicating potential roles in inflammation.
复制标题
呼吸机引起的肺损伤小鼠模型中长非编码 RNA 的表达谱分析表明其在炎症中的潜在作用。
DOI：
10.1002/jcb.28446
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发表时间：
2019-07
影响因子：
4
通讯作者：
Zhan QY
中科院分区：
文献类型：
--
作者：
Zhang NN;Zhang Y;Wang L;Xia JG;Liang ST;Wang Y;Wang ZZ;Huang X;Li M;Zeng H;Zhan QY
呼吸机诱导的肺损伤（VILI）炎症的关键调节因子仍然不明确。长链非编码rna （lncRNAs）与许多疾病的炎症反应有关；然而，它们在VILI中的作用仍不清楚。因此，我们利用RNA测序对VILI小鼠模型和对照组小鼠肺部的lncRNA和信使RNA （mRNA）进行转录组分析。通过RNA测序和定量逆转录聚合酶链反应分析基因表达。通过综合生物信息学分析，对不同时间点的对照组和损伤模型的表达谱和相关生物学功能进行了表征，并进行了多重比较。最后，构建lncRNA - mRNA共表达网络，并对失调的lncRNA进行功能分析。mRNA转录谱分析、共表达网络分析和功能分析表明，改变的lncRNAs在免疫系统/炎症过程、应激反应和炎症途径的调节中富集。我们发现lncRNA Gm43181可能通过趋化因子受体趋化因子（C‐X‐C）受体2与肺损伤和中性粒细胞活化有关。总之，我们的研究提供了与VILI炎症相关的异常lncRNA改变的鉴定，并且lncRNA介导的调节模式可能有助于VILI炎症。我们利用RNA测序对呼吸机诱导肺损伤（VILI）小鼠模型和对照组的肺进行了长链非编码RNA （lncRNA）和信使RNA的转录组分析。我们的研究提供了与VILI炎症相关的异常lncRNA改变的鉴定，并且lncRNA介导的调节模式可能有助于VILI炎症。
The key regulators of inflammation underlying ventilator‐induced lung injury (VILI) remain poorly defined. Long noncoding RNAs (lncRNAs) have been implicated in the inflammatory response of many diseases; however, their roles in VILI remain unclear. We, therefore, performed transcriptome profiling of lncRNA and messenger RNA (mRNA) using RNA sequencing in lungs collected from mice model of VILI and control groups. Gene expression was analyzed through RNA sequencing and quantitative reverse transctiption polymerase chain reaction. A comprehensive bioinformatics analysis was used to characterize the expression profiles and relevant biological functions and for multiple comparisons among the controls and the injury models at different time points. Finally, lncRNA‐mRNA coexpression networks were constructed and dysregulated lncRNAs were analyzed functionally. The mRNA transcript profiling, coexpression network analysis, and functional analysis of altered lncRNAs indicated enrichment in the regulation of immune system/inflammation processes, response to stress, and inflammatory pathways. We identified the lncRNA Gm43181 might be related to lung damage and neutrophil activation via chemokine receptor chemokine (C‐X‐C) receptor 2. In summary, our study provides an identification of aberrant lncRNA alterations involved in inflammation upon VILI, and lncRNA‐mediated regulatory patterns may contribute to VILI inflammation. We performed transcriptome profiling of long noncoding RNA (lncRNA) and messenger RNA using RNA sequencing in lungs collected from mice model of the ventilator‐induced lung injury (VILI) and control groups. Our study provides an identification of aberrant lncRNA alterations involved in inflammation upon VILI, and lncRNA‐mediated regulatory patterns may contribute to VILI inflammation.
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影响因子：
8.8
作者：
dos Santos, Claudia C.;Okutani, Daisuke;Liu, Mingyao
通讯作者：
Liu, Mingyao
DOI：
10.1164/ajrccm.163.3.2003001
发表时间：
2001-03-01
影响因子：
24.7
作者：
Held, HD;Boettcher, S;Uhlig, S
通讯作者：
Uhlig, S
影响因子：
64.5
作者：
Atianand MK;Hu W;Satpathy AT;Shen Y;Ricci EP;Alvarez-Dominguez JR;Bhatta A;Schattgen SA;McGowan JD;Blin J;Braun JE;Gandhi P;Moore MJ;Chang HY;Lodish HF;Caffrey DR;Fitzgerald KA
通讯作者：
Fitzgerald KA
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
8.8
作者：
Gajic, O;Dara, SI;Hubmayr, RD
通讯作者：
Hubmayr, RD