THE ROLE OF HISTONE-H1 AND NON-STRUCTURED DOMAINS OF CORE HISTONES IN MAINTAINING THE ORIENTATION OF NUCLEOSOMES WITHIN THE CHROMATIN FIBER
THE ROLE OF HISTONE-H1 AND NON-STRUCTURED DOMAINS OF CORE HISTONES IN MAINTAINING THE ORIENTATION OF NUCLEOSOMES WITHIN THE CHROMATIN FIBER
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DOI：
10.1016/0006-291x(84)91193-8
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
PASHEV, IG
中科院分区：
文献类型：
--
作者：
MAKAROV, VL;DIMITROV, SI;PASHEV, IG
用胰蛋白酶消化小牛胸腺染色质，发生的结构改变随后出现流线性二色性。在初始急剧增加之后，正信号的幅度逐渐下降，随后二色性符号发生变化，并且负信号进一步增加直至稳定。将二色性的这些变化与组蛋白模式的相应变化进行比较。染色质的正二色性不依赖于染色质的浓缩状态，核小体沿染色质纤维的方向由 H1 的球状结构域和核心组蛋白的非结构部分维持。
Calf thymus chromatin was digested with trypsin and the structural alterations which occurred were followed by flow linear dichroism. After a sharp initial increase, the amplitude of the positive signal gradually decreased followed by a change of the sign of the dichroism and further increase of the negative signal up to a plateau. These changes of the dichroism were compared to the respective changes in the histone pattern. The positive dichroism of chromatin did not depend on the condensation state of chromatin and the orientation of the nucleosomes along the chromatin fiber was maintained by the globular domain of H1 and the non-structured parts of core histones.