Production and characterization of murine monoclonal antibodies specific for baboon IgG heavy and light chain epitopes.
Production and characterization of murine monoclonal antibodies specific for baboon IgG heavy and light chain epitopes.
复制标题
狒狒 IgG 重链和轻链表位特异性鼠单克隆抗体的生产和表征。
DOI：
10.1111/j.1600-0684.1994.tb00124.x
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发表时间：
1994
影响因子：
0.7
通讯作者：
Kennedy,RC
中科院分区：
文献类型：
--
作者：
Shearer,MH;Jenson,HB;Carey,KD;Chanh,TC;Kennedy,RC
我们已经生成了一组识别狒狒IgG表位的小鼠单克隆抗体（mab）。同时还检测了单克隆抗体与其他灵长类动物IgG的反应性。IgG重(H)链或轻(L)链表位的特异性采用Western blot分析。分析了H链特异性单克隆抗体对IgG亚类的特异性和L链特异性单克隆抗体对狒狒IgM和聚合sIgA的反应性。最后，开发了ELISA来证明单克隆抗体在分析狒狒体液免疫应答中的效用。
We have generated a panel of murine monoclonal antibodies (MAbs) that recognize baboon IgG epitopes. The reactivity of the MAbs with IgG from other primate species was also examined. Specificity for IgG heavy (H) or light (L) chain epitopes was determined by Western blot analysis. The H chain‐specific MAbs were analyzed for IgG subclass specificity and the L chain‐specific MAbs for reactivity with baboon IgM and polymeric sIgA. Finally, an ELISA was developed to demonstrate the utility of the MAbs in analysis of humoral immune responses in baboons.