Peptides associated with eggs: mechanisms of interaction with spermatozoa.
Peptides associated with eggs: mechanisms of interaction with spermatozoa.
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与卵子相关的肽：与精子相互作用的机制。
DOI：
10.1007/978-1-4613-2255-9_18
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发表时间：
1986
影响因子：
--
通讯作者：
Thorpe,D
中科院分区：
文献类型：
--
作者：
Garbers,DL;Bentley,JK;Dangott,LJ;Ramarao,CS;Shimomura,H;Suzuki,N;Thorpe,D
精子肽（Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly）是从紫棘球蚴卵培养液中提取的一种肽，在适当条件下，能刺激紫棘球蚴精子的呼吸和运动。Resact（Cys-Val-Thr-Gly- Ala-Pro-Gly-Cys-Val-Gly-Gly-Gly-Arg-LeuNH_2）是一种从Arbaciapunctulata卵中提取的多肽，它也能刺激精子的代谢和运动，但不能刺激紫孢酵母精子。精子对卵肽的早期生化反应包括净H+流出和环AMP和环GMP浓度升高。此外，在点状曲霉精子中，一种主要的质膜蛋白响应于反应而被修饰，使得其表观分子量从160，000变为150，000。如果细胞与32 P一起孵育，160，000分子量的蛋白质被放射性标记;随后加入反应物会导致32 P从蛋白质中迅速丢失。质膜蛋白似乎是酶，鸟苷酸环化酶;与表观分子量的变化一致，酶活性降低高达90%。由于精子不能引起斑点曲霉的这些反应，因此可以得出结论，这些事件是受体介导的。
Speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly), a peptide obtained from the culture medium ofStrongylocentrotuspurpuratuseggs, stimulates the respiration and motility ofS.purpuratusspermatozoa under appropriate conditions. Resact (Cys-Val-Thr-Gly- Ala-Pro-Gly-Cys-Val-Gly-Gly-Gly-Arg-LeuNH2), a peptide obtained fromArbaciapunctulataeggs also stimulates the metabolism and motility ofA.punctulataspermatozoa, however, it fails to stimulateS.purpuratusspermatozoa. Early biochemical responses of the spermatozoa to the egg peptides include a net H+efflux and elevations of cyclic AMP and cyclic GMP concentrations. In addition, inA.punctulataspermatozoa, a major plasma membrane protein is modified in response to resact such that its apparent molecular weight shifts from 160,000 to 150,000. If cells are incubated with32P, the 160,000 molecular weight form of the protein becomes radiolabeled; subsequent addition of resact causes a rapid loss of32P from the protein. The plasma membrane protein appears to be the enzyme, guanylate cyclase; coincident with the shift in apparent molecular weight, enzyme activity decreases by as much as 90%. Since speract fails to cause these responses inA.punctulata, it can be concluded that the events are receptor-mediated.