OXYSTEROL-INDUCED APOPTOSIS IN HUMAN MONOCYTIC CELL-LINES
OXYSTEROL-INDUCED APOPTOSIS IN HUMAN MONOCYTIC CELL-LINES
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DOI：
10.1016/s0171-2985(11)80108-7
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发表时间：
1995-11-01
期刊：
影响因子：
2.8
通讯作者：
BISCHOFF, P
中科院分区：
文献类型：
--
作者：
AUPEIX, K;WELTIN, D;BISCHOFF, P
氧化甾醇是一大类天然化合物，具有调节胆固醇、免疫抑制和抗肿瘤等多种生物活性。在本研究中，我们检测和比较了该家族中具有代表性的两个成员：7β-羟基胆固醇(7β-OH)和25-羟基胆固醇(25-OH)对两种人单核细胞系U-937和HL-60的细胞毒作用。在两种细胞系中，7β-OH在30 mM时，在处理的第一个小时内就会通过细胞凋亡诱导细胞死亡。在相同的条件下，与以前在淋巴瘤细胞上获得的结果相反，25-OH仅具有细胞抑制作用。值得注意的是，用等摩尔浓度的7β-羟基和25-羟基同时处理U-937细胞，可显著降低细胞凋亡的诱导率。在HL-60细胞中没有观察到这样的影响。综上所述，这些结果首次表明：1)固醇核上羟化的氧合甾醇也能够诱导细胞凋亡，2)这些物质可以诱导髓系细胞的凋亡，3)就细胞凋亡而言，7β-OH和25-OH的联合处理可能会根据细胞类型的不同而导致相反的作用。
Oxysterols constitute a large family of natural compounds, endowed with various biological activities including cholesterol regulation, immunosuppression and antitumoral potency. In the present study, we examine and compare the cytotoxic effects of two representative members of this family: 7 beta-hydroxycholesterol (7 beta-OH) and 25-hydroxycholesterol (25-OH), in two human monocytic cell lines, U-937 and HL-60. In both cell lines 7 beta-OH at 30 mu M induces cell death by apoptosis within the first hours of treatment. Under the same conditions and in contrast with results previously obtained with lymphoma cells, 25-OH is cytostatic only. It is interesting to note that the simultaneous treatment of U-937 cells by equimolar concentrations of 7 beta-OH and 25-OH leads to a considerably decreased induction of apoptosis. Such an effect is not observed with HL-60 cells. Taken together, these results indicate for the first rime that: 1) oxysterols hydroxylated on the sterol nucleus are also able to induce apoptosis, 2) apoptosis can be induced by these substances in cells belonging to the myeloid lineage and 3) as far as apoptosis is concerned, a combined treatment with 7 beta-OH and 25-OH can lead to opposite effects depending on the cell type.