Crystallization and preliminary X-ray analysis of the low molecular weight phosphotyrosyl protein phosphatase from bovine heart.
Crystallization and preliminary X-ray analysis of the low molecular weight phosphotyrosyl protein phosphatase from bovine heart.
复制标题
牛心脏低分子量磷酸酪氨酰蛋白磷酸酶的结晶和初步 X 射线分析。
DOI：
10.1006/jmbi.1994.1287
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发表时间：
1994
影响因子：
5.6
通讯作者：
Stauffacher,CV
中科院分区：
文献类型：
--
作者：
Zhang,M;VanEtten,RL;Lawrence,CM;Stauffacher,CV
用X射线衍射法研究了牛心磷酸酪氨酰蛋白磷酸酶（BHPTP）的两种晶型。一种晶体形式生长为具有三斜晶体对称性的长棒，并衍射至3 μ m分辨率。这种晶体的衍射图显示出围绕长轴的孪晶。BHPTP的第二种晶型生长为具有单斜晶系的平面梯形棱柱，晶胞参数a = 95·3 °，B= 43·3 °，c= 41·2 °，β = 113·5°。晶胞尺寸表明每个不对称单位有一个18 kDa分子。这些晶体的分辨率至少为2.2 μ m，并且能够抵抗X射线束的衰变。
Two crystal forms of bovine heart phosphotyrosyl protein phosphatase (BHPTP) have been examined by X-ray analysis. One crystal form grows as long rods with triclinic crystal symmetry and diffracts to 3 Å resolution. The diffraction pattern of this form of the crystal shows twinning about a major axis. A second crystal form of BHPTP grows as flat trapezoidal prisms with monoclinic symmetryC2, and unit cell parametersa= 95·3 Å,b= 43·3 Å,c= 41·2 Å and β = 113·5°. The unit cell dimensions indicate that there is one 18 kDa molecule per asymmetric unit. These crystals diffract to at least 2·2 Å resolution and are resistant to decay in the X-ray beam.