CULTIVATION OF THE DROSOPHILA SEX-RATIO SPIROPLASMA

CULTIVATION OF THE DROSOPHILA SEX-RATIO SPIROPLASMA
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DOI:
10.1126/science.232.4755.1253
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发表时间:
1986-06-06
期刊:
影响因子:
56.9
通讯作者:
WHITCOMB, RF
WHITCOMB, RF
中科院分区:
综合性期刊1区
文献类型:
--
作者:
HACKETT, KJ;LYNN, DE;WHITCOMB, RF

文献摘要

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不可培养超过25年,在含有胚胎来源鳞翅目细胞系(IPLB-TN-R2)的组织培养基(H-2)中培养了willlistoni性别比例螺原体。适应后,在无细胞的H-2培养基中生长。这一成功证明了细胞培养系统对培养挑剔微生物的有用性,并促进了果蝇性别比例性状的研究。
Uncultivable for more than 25 years, the sex-ratio spiroplasma of Drosophila willistoni grew in a tissue culture medium (H-2) containing an embryo-derived lepidopteran cell line (IPLB-TN-R2). After adaptation, it grew in a cell-free H-2 medium. This success demonstrates the usefulness of cell culture systems for cultivation of fastidious microorganisms and facilitates study of the sex-ratio trait in Drosophila.