Imaging the distribution of individual platinum-based anticancer drug molecules attached to single-wall carbon nanotubes.
Imaging the distribution of individual platinum-based anticancer drug molecules attached to single-wall carbon nanotubes.
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DOI：
10.2217/nnm.09.56
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Rusling JF
中科院分区：
文献类型：
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作者：
Bhirde AA;Sousa AA;Patel V;Azari AA;Gutkind JS;Leapman RD;Rusling JF
成像附着在单壁碳纳米管（SWNTs）上的药物分子的分布。在此，我们报告了使用扫描透射电子显微镜（STEM）对附着在swnt上的单个铂基药物分子进行原子尺度的可视化和定量。swnt是为靶向药物递送而设计的。傅里叶变换红外光谱和能量色散x射线光谱对SWNT药物偶联物进行了表征。Z-contrast STEM成像使一线抗癌药物顺铂在纳米管上的单分子水平可视化。利用能量色散x射线光谱和傅里叶变换红外光谱证实了顺铂在纳米管上的存在。STEM断层扫描也用于提供有关纳米管共轭物的额外见解。最后，我们的观察结果为探索使用SWNT生物偶联物选择性靶向和杀死鳞状癌细胞提供了理论依据。z对比STEM成像提供了一种直接可视化附着在碳单壁碳纳米管表面的重金属分子（即顺铂）以及分布和定量的手段。
To image the distribution of drug molecules attached to single-wall carbon nanotubes (SWNTs). Herein we report the use of scanning transmission electron microscopy (STEM) for atomic scale visualization and quantitation of single platinum-based drug molecules attached to SWNTs designed for targeted drug delivery. Fourier transform infrared spectroscopy and energy-dispersive x-ray spectroscopy were used for characterization of the SWNT drug conjugates. Z-contrast STEM imaging enabled visualization of the first-line anticancer drug cisplatin on the nanotubes at single molecule level. The identity and presence of cisplatin on the nanotubes was confirmed using energy-dispersive x-ray spectroscopy and Fourier transform infrared spectroscopy. STEM tomography was also used to provide additional insights concerning the nanotube conjugates. Finally, our observations provide a rationale for exploring the use of SWNT bioconjugates to selectively target and kill squamous cancer cells. Z-contrast STEM imaging provides a means for direct visualization of heavy metal containing molecules (i.e., cisplatin) attached to surfaces of carbon SWNTs along with distribution and quantitation.