Hybrid Erythrocyte Liposomes: Functionalized Red Blood Cell Membranes for Molecule Encapsulation
Hybrid Erythrocyte Liposomes: Functionalized Red Blood Cell Membranes for Molecule Encapsulation
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DOI：
10.1002/adbi.201900185
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发表时间：
2020-01-07
影响因子：
4.1
通讯作者：
Rheinstadter, Maikel C.
中科院分区：
文献类型：
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作者：
Himbert, Sebastian;Blacker, Matthew J.;Rheinstadter, Maikel C.
红细胞膜性质的修饰为改善药物传递和生物医学应用提供了新的工具。通过在红细胞膜上掺杂不同类别（PC， PS， PG）和不同饱和度（14:0,16:0-18:1）的合成脂质分子，制备了杂交红细胞脂质体。确定了各自的溶解度限制，并通过x射线衍射、外延荧光显微镜、动态光散射（DLS）、Zeta电位、紫外-可见光谱和分子动力学（MD）模拟研究了混合脂质体的材料性质。膜厚度和脂质取向可以通过添加磷脂酰胆碱脂来调节。混合膜可以通过加入德克萨斯红DHPE进行荧光标记，并通过加入磷脂酰丝氨酸和磷脂酰甘油对其电荷进行修饰。以荧光素标记的葡聚糖为例，证明了小分子可以被封装到这些杂交脂质体中。
The modification of erythrocyte membrane properties provides a new tool towards improved drug delivery and biomedical applications. The fabrication of hybrid erythrocyte liposomes is presented by doping red blood cell membranes with synthetic lipid molecules of different classes (PC, PS, PG) and different degrees of saturation (14:0, 16:0-18:1). The respective solubility limits are determined, and material properties of the hybrid liposomes are studied by a combination of X-ray diffraction, epi-fluorescent microscopy, dynamic light scattering (DLS), Zeta potential, UV-vis spectroscopy, and Molecular Dynamics (MD) simulations. Membrane thickness and lipid orientation can be tuned through the addition of phosphatidylcholine lipids. The hybrid membranes can be fluorescently labelled by incorporating Texas-red DHPE, and their charge modified by incorporating phosphatidylserine and phosphatidylglycerol. By using fluorescein labeled dextran as an example, it is demonstrated that small molecules can be encapsulated into these hybrid liposomes.