Method of imaging low density lipoproteins by atomic force microscopy
Method of imaging low density lipoproteins by atomic force microscopy
复制标题
DOI：
10.1002/jemt.20492
复制
发表时间：
2007-10-01
影响因子：
2.5
通讯作者：
Vermette, Patrick
中科院分区：
文献类型：
--
作者：
Chouinard, Julie A.;Khalil, Abdelouahed;Vermette, Patrick
本文介绍了一种利用原子力显微镜（AFM）对低密度脂蛋白（LDL）进行成像的简单方法。该仪器允许对液体中的生物样品进行成像，并且呈现出不需要样品制备（诸如可能影响其一般结构的染色或固定）的优点。成功测量了单个LDL颗粒的尺寸（直径和高度）。AFM成像显示LDL在x和y轴上具有准球形结构，在z轴上具有扁球体结构（即，高度）。发现LDL具有23 +/-3 nm的平均直径。获得的平均高度为10 +/-2 nm。
This short paper reports a simple method to image low density lipoproteins (LDL) using atomic force microscopy (AFM). This instrument allows imaging of biological samples in liquid and presents the advantage of needing no sample preparation such as staining or fixation that may affect their general structure. Dimensions (diameter and height) of individual LDL particles were successfully measured. AFM imaging revealed that LDL have a quasi-spherical structure on the x and y axis with an oblate spheroid structure in the z axis (i.e., height). LDLs were found to have an average diameter of 23 +/- 3 nm. The obtained mean height was 10 +/- 2 nm.