High-resolution genotyping of Chlamydia trachomatis strains by multilocus sequence analysis
High-resolution genotyping of Chlamydia trachomatis strains by multilocus sequence analysis
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DOI：
10.1128/jcm.02301-06
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发表时间：
2007-05-01
影响因子：
9.4
通讯作者：
Herrmann, Bjorn
中科院分区：
文献类型：
--
作者：
Klint, Markus;Fuxelius, Hans-Henrik;Herrmann, Bjorn
沙眼衣原体的基因分型受限于基因组中的低序列变异，并且没有足够的方法可用于分析衣原体感染在社区中的传播。我们已经开发了一个多位点序列分型（MLST）系统的基础上，五个目标区域，并比较它与ompA，最广泛用于基因分型的单基因的分析。对16种参考菌株（包括所有主要血清型，血清型A至L3）进行的序列测定显示，5个独立靶区域中的遗传变异数范围为8至16。对47株临床分离的C.分析了代表性血清型的沙眼分离株（14个血清型D、12个血清型E、11个血清型G和10个血清型K菌株）; MLST系统检测到32个变体，而使用ompA分析检测到12个变体。主要血清型，血清型E的标本，分为7种基因型MLST，但只有两个ompA分析。将MLST系统应用于C.来自男男性行为人群的沙眼标本，并能够将一种主要ompA基因型G变异体的10份标本区分为4种不同的MLST变异体。总之，我们的MLST系统可以用来区分C。可应用于高分辨率的分子流行病学。
Genotyping of Chlamydia trachomatis is limited by the low sequence variation in the genome, and no adequate method is available for analysis of the spread of chlamydial infections in the community. We have developed a multillocus sequence typing (MLST) system based on five target regions and compared it with analysis of ompA, the single gene most extensively used for genotyping. Sequence determination of 16 reference strains, comprising all major serotypes, serotypes A to L3, showed that the number of genetic variants in the five separate target regions ranged from 8 to 16. The genetic variation in 47 clinical C. trachomatis isolates of representative serotypes (14 serotype D, 12 serotype E, 11 serotype G, and 10 serotype K strains) was analyzed; and the MLST system detected 32 variants, whereas 12 variants were detected by using ompA analysis. Specimens of the predominant serotype, serotype E, were differentiated into seven genotypes by MLST but into only two by ompA analysis. The MLST system was applied to C. trachomatis specimens from a population of men who have sex with men and was able to differentiate 10 specimens of one predominant ompA genotype G variant into four distinct MLST variants. To conclude, our MLST system can be used to discriminate C. trachomatis strains and can be applied to high-resolution molecular epidemiology.