CLONING THE DIFFERENCES BETWEEN 2 COMPLEX GENOMES
CLONING THE DIFFERENCES BETWEEN 2 COMPLEX GENOMES
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DOI：
10.1126/science.8438152
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发表时间：
1993-02-12
期刊：
影响因子：
56.9
通讯作者：
WIGLER, M
中科院分区：
文献类型：
--
作者：
LISITSYN, N;LISITSYN, N;WIGLER, M
对两个复杂基因组之间差异的分析有望发现对遗传研究有用的感染因子和探针。开发了一种系统，其中使用消减和动力学富集来纯化存在于一个DNA片段群体中而不存在于另一个群体中的限制性内切酶片段。将该方法应用于复杂性降低的DNA群体（“代表”）导致分离出在人类DNA中以单拷贝存在的病毒基因组的探针，以及检测两个个体之间多态性的探针。原则上，这种被称为代表性差异分析（RDA）的系统也可用于分离与基因组重排位点相连的探针，无论是自发发生并导致遗传性疾病或癌症，还是在分化和发育期间编程。
The analysis of the differences between two complex genomes holds promise for the discovery of infectious agents and probes useful for genetic studies. A system was developed in which subtractive and kinetic enrichment was used to purify restriction endonuclease fragments present in one population of DNA fragments but not in another.Application of this method to DNA populations of reduced complexity (''representations'') resulted in the isolation of probes to viral genomes present as single copies in human DNA, and probes that detect polymorphisms between two individuals. In principle, this system, called representational difference analysis (RDA), may also be used for isolating probes linked to sites of genomic rearrangements, whether occurring spontaneously and resulting in genetic disorders or cancer, or programmed during differentiation and development.