The mouse cornea as a transplantation site for live imaging of engineered tissue constructs.
The mouse cornea as a transplantation site for live imaging of engineered tissue constructs.
复制标题
小鼠角膜作为移植部位，用于工程组织结构的实时成像。
DOI：
10.1101/pdb.prot5416
复制
发表时间：
2010
影响因子：
--
通讯作者：
Dickinson,MaryE
中科院分区：
文献类型：
--
作者：
Poché,RossA;Saik,JenniferE;West,JenniferL;Dickinson,MaryE
组织工程领域的目标是在体外重现健康的人体器官和3-D组织结构，然后将这些结构移植到体内，在那里它们可以有效地整合在受体患者体内，并通过宿主循环进行灌注。为了改进人工组织支架材料的设计，理想的做法是拥有一种高通量成像系统，使人们能够在很长一段时间内直接监测活体动物体内移植的组织结构。通过将这种实验与转基因的、细胞特异性的荧光报告相结合，可以监测诸如组织构建灌注、供体细胞存活和供体-宿主细胞相互作用/整合等参数。在这里，我们描述了一种经典角膜微袋血管生成试验的改进版本的方案，将其作为实时成像“窗口”来监测血管生成聚乙二醇（PEG）基水凝胶组织结构。
The field of tissue engineering aims to recapitulate healthy human organs and 3-D tissue structures in vitro and then transplant these constructs in vivo where they can be effectively integrated within the recipient patient and become perfused by the host circulation. To improve the design of materials for artificial tissue scaffolds, it would be ideal to have a high-throughput imaging system that allows one to directly monitor transplanted tissue constructs in live animals over an extended period of time. By combining such an assay with transgenic, cell-specific fluorescent reporters, one could monitor such parameters as tissue construct perfusion, donor cell survival, and donor-host cell interaction/integration. Here, we describe a protocol for a modified version of the classical corneal micropocket angiogenesis assay, employing it as a live imaging “window” to monitor angiogenic poly (ethylene glycol)(PEG)-based hydrogel tissue constructs.