NiO nanoparticle-based urea biosensor
NiO nanoparticle-based urea biosensor
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DOI：
10.1016/j.bios.2012.07.062
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发表时间：
2013-03-15
影响因子：
12.6
通讯作者：
Gupta, Vinay
中科院分区：
文献类型：
--
作者：
Tyagi, Manisha;Tomar, Monika;Gupta, Vinay
NiO纳米颗粒（NiO- nps）已被成功地用于制造尿素生物传感器。NiO纳米颗粒薄膜沉积在氧化铟锡（ITO）涂层的玻璃衬底上，作为固定脲酶（Ur）的有效基质，脲酶是尿素检测的特定酶。制备的生物电极（Ur/NiO-NP/ITO/玻璃）利用循环伏安法和紫外可见光谱法检测尿素。NiO纳米粒子作为电催化物质，基于八面体中Ni2+和Ni3+之间的电子穿梭，导致增强的电化学电流响应。所制备的生物电极（Ur/NiO-NPs/ITO/glass）具有21.3 μ a /(mM * cm(2))的高灵敏度，在尿素浓度范围（0.83-16.65 mM）内具有良好的线性关系，响应时间为5 s。Michaelis-Menten常数较低（K-m=0.34 mM）表明Ur对分析物（尿素）具有较高的亲和力。高催化活性，以及NiO-NPs的氧化还原行为，使其成为实现尿素生物传感器的有效基质。(C) 2012 Elsevier B.V.版权所有
NiO nanoparticles (NiO-NPs) have been exploited successfully for the fabrication of a urea biosensor. A thin film of NiO nanoparticles deposited on an indium tin oxide (ITO) coated glass substrate serves as an efficient matrix for the immobilisation of urease (Ur), the specific enzyme for urea detection. The prepared bioelectrode (Ur/NiO-NP/ITO/glass) is utilised for urea sensing using cyclic voltammetry and UV-visible spectroscopy. NiO nanoparticles act as electro-catalytic species that are based on the shuttling of electrons between Ni2+ and Ni3+ in the octahedral site and result in an enhanced electrochemical current response. The prepared bioelectrode (Ur/NiO-NPs/ITO/glass) exhibits a high sensitivity of 21.3 mu A/(mM * cm(2)) and a good linearity in a wide range (0.83-16.65 Mm) of urea concentrations with fast response time of 5 s. The low value of the Michaelis-Menten constant (K-m=0.34 mM) indicates the high affinity of Ur towards the analyte (urea). The high catalytic activity, along with the redox behaviour of NiO-NPs, makes it an efficient matrix for the realisation of a urea biosensor. (C) 2012 Elsevier B.V. All rights reserved.