From proteins to proteomes: Large scale protein identification by two-dimensional electrophoresis and amino acid analysis
From proteins to proteomes: Large scale protein identification by two-dimensional electrophoresis and amino acid analysis
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DOI：
10.1038/nbt0196-61
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Hochstrasser, DF
中科院分区：
文献类型：
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作者：
Wilkins, MR;Pasquali, C;Hochstrasser, DF
蛋白质的双向电泳分离和鉴定可用于基于蛋白质的基因表达分析。coli 2-D凝胶，通过与所有E.大肠杆菌的数据。30种蛋白质来自E. coli 2-D图谱分析和鉴定。其中三种蛋白质未知。通过蛋白质测序分析，27种蛋白质中有20种被正确鉴定，重要的是，正确的鉴定显示了明确的“正确”评分模式。而不正确的蛋白质鉴定也显示出独特的评分模式，表明蛋白质必须通过其他方法鉴定。这些技术允许大规模筛选简单生物体或处于正常和疾病状态的组织的蛋白质补体。本文所述的计算机程序可通过万维网在URL地址（http：//expasy.hcuge.ch/）处获得。
Separation and identification of proteins by two-dimensional (2-D) electrophoresis can be used for protein-based gene expression analysis, In this report single protein spots, from polpvinylidene difluoride blots of micropreparative E. coli 2-D gels, were rapidly and economically identified by matching their amino acid composition, estimated pi and molecular weight against all E. coli entries in the SWISS-PROT database. Thirty proteins from an E. coli 2-D map were analyzed and identities assigned. Three of the proteins were unknown. By protein sequencing analysis, 20 of the 27 proteins were correctly identified, Importantly, correct identifications showed unambiguous ''correct'' score patterns. While incorrect protein identifications also showed distinctive score patterns, indicating that protein must be identified by other means. These techniques allow large-scale screening of the protein complement of simple organisms, or tissues in normal and disease states, The computer program described here is accessible via the World Wide Web at URL address (http://expasy.hcuge.ch/).