Prevalence and Risk Factor Analysis of Bovine Hemoplasma Infection by Direct PCR in Eastern Hokkaido, Japan
Prevalence and Risk Factor Analysis of Bovine Hemoplasma Infection by Direct PCR in Eastern Hokkaido, Japan
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DOI：
10.1292/jvms.12-0118
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发表时间：
2012-09-01
影响因子：
1.2
通讯作者：
Inokuma, Hisashi
中科院分区：
文献类型：
--
作者：
Tagawa, Michihito;Ybanez, Adrian P.;Inokuma, Hisashi
采用直接PCR法对日本北海道东部343头牛的全血样品进行了2种牛血浆体--文氏支原体（Mw）和“红毛支原体”（CMh）的流行率和危险因素的检测。直接PCR法的灵敏度比标准PCR法高10倍，每次反应检测5个拷贝的牛血浆DNA。对采样地点、居住条件、年龄、牛白血病病毒抗体等进行流行病学调查，以评估感染的危险因素。Mw和CMh的患病率分别为38.5%和39.1%，所有牛血浆感染的总体患病率为64.7%。不同采样地点、居住环境、条件和年龄组间存在显著性差异。与黄牛相比，养殖牛对总的血浆菌感染的敏感性显著增高（P
The prevalence and risk factors of 2 bovine hemoplasma species-Mycoplasma wenyonii (Mw) and "Candidatus Mycoplasma haemobos" (CMh)-were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living, conditions and age. Compared with pastured cattle, farmed cattle were significantly more susceptible to infection with total hemoplasma species (P