Massive induction of donor-type class I and class II major histocompatibility complex antigens in rejecting cardiac allografts in the rat.
Massive induction of donor-type class I and class II major histocompatibility complex antigens in rejecting cardiac allografts in the rat.
复制标题
在拒绝大鼠心脏同种异体移植时，大量诱导了供体类型的I类和II类主要的组织相容性复合物。
DOI：
10.1084/jem.161.1.98
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发表时间：
1985-01-01
影响因子：
15.3
通讯作者：
Fabre, J W
中科院分区：
文献类型：
--
作者：
Milton, A D;Fabre, J W
DA（RT 1a）心脏移植到PVG（RT 1c）或DA受体，切除移植后1，3，5，或7天，并通过免疫组织化学技术和定量吸收分析，使用同种异体小鼠抗大鼠I类和II类主要组织相容性复合物（MHC）单克隆抗体进行检查。低温切片染色的过氧化物酶技术表明，在正常心脏，I类抗原主要限于血管内皮细胞和间质细胞，除了在闰盘没有观察到染色的心肌细胞。II类抗原只发现偶尔间质树突状细胞。移植后第1天的图像没有明显不同。然而，到第3天，心肌细胞上的I类和II类抗原都有明显的斑片状诱导，通常在细胞浸润区域。到第5天，I类抗原在整个移植物中被强烈诱导，心肌细胞呈非常强的阳性。在第5天，心肌细胞上也均匀地表达II类抗原，并且在此阶段血管内皮也呈强阳性。定量吸收分析显示，与正常DA心脏相比，移植后第5天心脏移植物中I类抗原含量增加了10倍。DA心脏同系移植物显示II类抗原没有增加，但有趣的是，移植后5天，似乎有一些I类抗原的心肌细胞上的表达。与正常DA心脏相比，5-D同种移植物的定量分析显示I类抗原增加了3倍。
DA (RT1a) hearts were transplanted into PVG (RT1c) or DA recipients, excised on days 1, 3, 5, or 7 after grafting, and examined by immunohistological techniques and quantitative absorption analyses, using allospecific mouse anti-rat class I and class II major histocompatibility complex (MHC) monoclonal antibodies. Cryostat sections stained by the peroxidase technique demonstrated that, in the normal heart, class I antigens were largely restricted to vascular endothelium and interstitial cells, with no observable staining of the myocardial cells except at the intercalated discs. Class II antigens were found only on occasional interstitial dendritic cells. The picture at day 1 after transplantation was not noticeably different. By day 3, however, there was clear patchy induction of both class I and class II antigens on the myocardial cells, usually in the region of cellular infiltrates. By day 5, class I antigens had been strongly induced throughout the graft, with the myocardial cells being very strongly positive. Class II antigens were also uniformly expressed on myocardial cells at day 5, and at this stage the vascular endothelium was also strongly positive. Quantitative absorption analyses showed a 10-fold increase in class I antigen content in cardiac allografts at day 5 after transplantation when compared with normal DA heart. DA heart isografts showed no increase in class II antigens, but it was interesting that, by 5 d after grafting, there appeared to be some expression of class I antigens on the myocardial cells. Quantitative absorptions showed a threefold increase in class I antigens on 5-d isografts when compared with normal DA heart.