Immunohistochemical analysis of lung carcinomas with pure or partial bronchioloalveolar differentiation.
Immunohistochemical analysis of lung carcinomas with pure or partial bronchioloalveolar differentiation.
复制标题
具有纯或部分细支气管肺泡分化的肺癌的免疫组织化学分析。
DOI：
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发表时间：
2004
影响因子：
4.6
通讯作者：
M. Fishbein
中科院分区：
文献类型：
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作者：
G. Sarantopoulos;D. Gui;P. Shintaku;Longsheng Hong;Ya;C. Yap;M. Fishbein
上下文
1999年，世界卫生组织将细支气管肺泡癌（BAC）重新定义为仅具有单纯的鳞片状生长模式且无侵袭性的肿瘤。
目标
本研究检查了45例具有BAC组分的肺癌，以确定这些肿瘤是否按照当前世界卫生组织标准被归类为BAC，（2）定量这些肿瘤中的BAC组分，以及（3）观察这些肿瘤的所谓侵袭性和非侵袭性区域之间是否存在表型差异。
设计
对苏木精-伊红染色切片进行回顾性审查，并对组织学分级、肿瘤亚型和纯BAC模式的百分比进行分类，通过甲状腺转录因子1、细胞角蛋白7、细胞角蛋白20和Ki-67抗体的免疫组化染色进行进一步表征。
结果
在45例肿瘤中，只有7例（15.6%）可按目前严格的世界卫生组织标准分类为BAC。这些肿瘤，被归类为非粘液性和混合性，表现出类似的免疫组化染色的细胞角蛋白7，细胞角蛋白20，甲状腺转录因子1，粘液性肿瘤表现出不同的染色。在指定为鳞片状、浸润性和前缘的肿瘤区域以及具有不同组织学分级（即，良好、中等和低分化）的肿瘤区域，观察到免疫组织化学染色和肿瘤细胞增殖的显著差异。
结论
非粘液性和混合性BAC在表型上相似，并显示相同的免疫组化染色模式;另一方面，粘液性肿瘤显示不同的免疫组化染色。肺肿瘤被称为具有BAC成分的腺癌，代表了具有一系列细胞类型、分化、生长和免疫表型的异质性组。在单个肿瘤中，这些参数也存在区域差异。
CONTEXT
In 1999, the World Health Organization redefined bronchioloalveolar carcinomas (BACs) as those neoplasms with only a pure lepidic growth pattern and no invasion.
OBJECTIVES
The present study examined 45 lung cancers with a BAC component (1) to determine whether these tumors would be classified as BACs by current World Health Organization standards, (2) to quantitate the BAC component within these tumors, and (3) to see if phenotypic differences exist between the so-called invasive and noninvasive regions of these tumors.
DESIGN
Retrospective review of hematoxylin-eosin-stained slides and classification of histologic grade, tumor subtype, and percentage of pure BAC pattern, with further characterization by immunohistochemical staining for thyroid transcription factor 1, cytokeratin 7, cytokeratin 20, and Ki-67 antibodies.
RESULTS
Only 7 (15.6%) of the 45 tumors examined could be classified as BAC by current strict World Health Organization criteria. Those tumors, classified as nonmucinous and mixed, showed similar immunohistochemical staining for cytokeratin 7, cytokeratin 20, and thyroid transcription factor 1; mucinous tumors showed disparate staining. Significant differences in immunohistochemical staining and tumor cell proliferation were seen for the regions of tumors designated as lepidic, infiltrative, and leading edge and for the regions of tumors with different histologic grades (ie, well, moderately, and poorly differentiated).
CONCLUSIONS
Nonmucinous and mixed BACs are phenotypically similar and show identical immunohistochemical staining patterns; mucinous tumors, on the other hand, show disparate immunohistochemical staining. Pulmonary neoplasms designated as adenocarcinomas with a BAC component represent a heterogenous group with a range of cell types, differentiation, growth, and immunophenotypes. Within an individual neoplasm, there are regional differences in these parameters as well.