Enhanced expression of glutamine synthetase (GS1a) confers altered fibre and wood chemistry in field grown hybrid poplar (Populus tremula X alba) (717-1B4)
Enhanced expression of glutamine synthetase (GS1a) confers altered fibre and wood chemistry in field grown hybrid poplar (Populus tremula X alba) (717-1B4)
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DOI：
10.1111/j.1467-7652.2012.00714.x
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发表时间：
2012-09-01
影响因子：
13.8
通讯作者：
Mansfield, Shawn D.
中科院分区：
文献类型：
--
作者：
Coleman, Heather D.;Canovas, Francisco M.;Mansfield, Shawn D.
通过基因工程改造表达松树胞质谷氨酰胺合成酶基因（GS1a）的杂交白杨（Populus tremula X P. alba）先前已显示出理想的田间表现特征，包括生长和氮利用效率的增强。木材样品从3岁的现场试验的三个独立的GS1a转基因杂交白杨系的分析表明，与野生型对照相比，GS1a的异位表达导致木材性质和木材化学的改变。包括木材纤维长度，木材密度，微纤维角度，%的双辛基木质素和木材糖，特别是葡萄糖，半乳糖，甘露糖和木糖的浓度升高显着增强。与野生型树木相比，GS1a转基因木材中的总提取物含量和酸不溶性木质素显着降低。总之，这些细胞壁特征导致改进的木材制浆属性，包括改进的木质素溶解，而不同时降低产率。GS1a表达增加的树木具有改善的纸浆和纸张生产特性，并具有作为生物燃料生产原料的潜力。
Hybrid poplar (Populus tremula X P. alba) genetically engineered to express the pine cytosolic glutamine synthetase gene (GS1a) has been previously shown to display desirable field performance characteristics, including enhancements in growth and nitrogen use efficiency. Analysis of wood samples from a 3-year-old field trial of three independently transformed GS1a transgenic hybrid poplar lines revealed that, when compared with wild-type controls, ectopic expression of GS1a resulted in alterations in wood properties and wood chemistry. Included were significant enhancements in wood fibre length, wood density, microfibre angle, per cent syringyl lignin and elevated concentrations of wood sugars, specifically glucose, galactose, mannose and xylose. Total extractive content and acid-insoluble lignin were significantly reduced in wood of GS1a transgenics when compared with wild-type trees. Together, these cell wall characteristics resulted in improved wood pulping attributes, including improved lignin solubilization with no concurrent decrease in yield. Trees with increased GS1a expression have improved characteristics for pulp and paper production and hold potential as a feedstock for biofuels production.