SONOMAGNETIC STIMULATION OF LIVE CELLS: ELECTROPHYSIOLOGIC, BIOCHEMICAL AND BEHAVIORAL RESPONSES
SONOMAGNETIC STIMULATION OF LIVE CELLS: ELECTROPHYSIOLOGIC, BIOCHEMICAL AND BEHAVIORAL RESPONSES
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DOI：
10.1016/j.ultrasmedbio.2019.07.009
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发表时间：
2019-11-01
影响因子：
2.9
通讯作者：
Chen, Siping
中科院分区：
文献类型：
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作者：
Hu, Yaxin;Wang, Yancheng;Chen, Siping
已经开发了各种物理方法来调节细胞的电生理特性及其生化信号通路。在这项研究中，我们提出了一种使用脉冲超声（1.1 MHz频率、1.1或2.2 MPa压力、每个脉冲50个周期和500 Hz脉冲重复频率）和静态磁场（680 mT）刺激活细胞的声磁方法。我们发现，声磁刺激促进细胞和线粒体膜电位更超极化状态。细胞膜超极化程度与细胞类型有关。此外，我们发现，细胞内的Ca 2+离子，活性氧和一氧化氮的浓度显着增加声磁刺激后，细胞内的pH值也观察到一个小的下降。最后，我们发现每天声磁刺激3d对neuro-2a癌细胞的增殖抑制率为48.64%。我们的工作表明，声磁刺激可以有效地扰乱细胞信号传导，并将癌细胞驱动到相对静止的状态。(C)2019年世界医学和生物学超声联合会。All rights reserved.
Various physical methods have been developed to modulate the electrophysiologic properties of cells and their biochemical signaling pathways. In this study, we propose a sonomagnetic method using pulsed ultrasound (1.1 MHz frequency, 1.1 or 2.2 MPa pressure, 50 cycles per pulse and 500 Hz pulse repetition frequency) and a static magnetic field (680 mT) to stimulate live cells. We found that sonomagnetic stimulation promoted the cell and mitochondrial membrane potentials to more hyperpolarized states. The degree of cell membrane hyperpolarization was cell-type dependent. Furthermore, we found that the intracellular concentrations of Ca2+ ions, reactive oxygen species and nitric oxide were substantially increased after sonomagnetic stimulation, and a small decrease in intracellular pH was also observed. Lastly, we found that the daily sonomagnetic stimulation for 3 d inhibited the proliferation rate of neuro-2a cancer cells by 48.64%. Our work demonstrates that sonomagnetic stimulation can effectively perturb cell signaling and drive cancer cells into relatively quiescent states. (C) 2019 World Federation for Ultrasound in Medicine & Biology. All rights reserved.