Chitosan primes plant defence mechanisms against Botrytis cinerea , including expression of Avr9/Cf-9 rapidly-elicited genes
Chitosan primes plant defence mechanisms against Botrytis cinerea , including expression of Avr9/Cf-9 rapidly-elicited genes
复制标题
壳聚糖启动植物防御灰葡萄孢的机制，包括 Avr9/Cf-9 快速引发基因的表达
DOI：
10.1101/2020.04.01.019513
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
De Vega D
中科院分区：
文献类型：
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作者：
De Vega D
目前的作物保护策略主要是传统杀菌剂和寄主遗传抗性的结合。然而，由于病原体的进化和使用杀菌剂的立法，这些策略不足以保护植物免受这种病原体的侵害。防御激发子可以通过一种被称为防御启动的现象刺激植物的防御机制。在病原体被宿主识别后，启动导致更快和/或更强的抗性表达。这项工作的目的是研究防御启动的商业配方引发剂壳聚糖。壳聚糖对茄科和十字花科植物具有诱导抗性。在番茄植株中，抗性的增强与胼胝质沉积的启动和植物激素茉莉酸（JA）的积累有关。大规模转录组学分析显示，壳聚糖在感染后的早期时间点启动基因表达。此外，还鉴定出两个具有特异引物特征的番茄新基因，Avr9/Cf‐9快速诱导蛋白75 （ACRE75）和180 （ACRE180）。facre75、acre180及其底栖烟草同源物的瞬时和稳定过表达表明，它们是植物对b抗性的正调控因子。灰质。这为寻找茄科植物的防治策略提供了有价值的信息。灰质。
Current crop protection strategies against the fungal pathogenBotrytis cinerearely on a combination of conventional fungicides and host genetic resistance. However, due to pathogen evolution and legislation in the use of fungicides, these strategies are not sufficient to protect plants against this pathogen. Defence elicitors can stimulate plant defence mechanisms through a phenomenon known as defence priming. Priming results in a faster and/or stronger expression of resistance upon pathogen recognition by the host. This work aims to study defence priming by a commercial formulation of the elicitor chitosan. Treatments with chitosan result in induced resistance (IR) in solanaceous and brassicaceous plants. In tomato plants, enhanced resistance has been linked with priming of callose deposition and accumulation of the plant hormone jasmonic acid (JA). Large‐scale transcriptomic analysis revealed that chitosan primes gene expression at early time‐points after infection. In addition, two novel tomato genes with a characteristic priming profile were identified, Avr9/Cf‐9 rapidly elicited protein 75 (ACRE75) and 180 (ACRE180). Transient and stable over‐expression ofACRE75,ACRE180and theirNicotiana benthamianahomologs, revealed that they are positive regulators of plant resistance againstB. cinerea.This provides valuable information in the search for strategies to protect Solanaceae plants againstB. cinerea.