Isolation and culture of rat and mouse oligodendrocyte precursor cells
Isolation and culture of rat and mouse oligodendrocyte precursor cells
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DOI：
10.1038/nprot.2007.149
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Lu, Q. Richard
中科院分区：
文献类型：
--
作者：
Chen, Ying;Balasubramaniyan, Veerakumar;Lu, Q. Richard
分离少突胶质细胞前体细胞 (OPC) 的能力为表征其分化、特性和髓磷脂修复潜力提供了强大的手段。尽管从大鼠中枢神经系统中分离 OPC 已有很多知识，但由于难以获得足够数量的纯化 OPC，因此小鼠 OPC 的制备和维护直到最近仍然是一个挑战。在这里，我们描述了制备高度富集的大鼠 OPC 和几乎同质的小鼠 OPC 的方案。小鼠方法利用分子遗传工具，主要从皮质神经祖细胞中产生称为“寡球”的克隆聚集体的 OPC。分离的 OPC 可以进一步分化为少突胶质细胞。总的来说，我们描述了从大鼠和小鼠中制备和体外维持富集 OPC 的简单有效的方法。大量同质啮齿动物 OPC 群体的分离和培养应显着促进 OPC 谱系进展及其在损伤后髓磷脂修复。
The ability to isolate oligodendroglial precursor cells ( OPCs) provides a powerful means to characterize their differentiation, properties and potential for myelin repair. Although much knowledge is available for isolation of OPCs from the rat central nervous system, preparation and maintenance of mouse OPCs has been until recently a challenge owing to difficulties in obtaining a sufficient quantity of purified OPCs. Here, we describe protocols to prepare highly enriched rat OPCs and nearly homogenous mouse OPCs. The mouse method generates predominantly OPCs from cortical neural progenitor cells as clonal aggregates called "oligospheres'' by taking advantage of molecular genetic tools. Isolated OPCs can be further differentiated into oligodendrocytes. Collectively, we describe simple and efficient methods for the preparation and in vitro maintenance of enriched OPCs from rats and mice. Isolation and culture of a large, homogenous population of rodent OPCs should significantly facilitate studies on OPC lineage progression and their utility in myelin repair after injury.