Prolongation of the effective copulation period by fractionated-dose irradiation in the sweet potato weevil, Cylas formicarius
Prolongation of the effective copulation period by fractionated-dose irradiation in the sweet potato weevil, Cylas formicarius
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分次剂量照射延长甘薯象甲Cylas formicarius的有效交配期
DOI：
10.1111/j.1570-7458.2011.01181.x
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发表时间：
2011
影响因子：
1.9
通讯作者：
Tsuguo Kohama
中科院分区：
文献类型：
--
作者：
Norikuni Kumano;Takashi Kuriwada;Keiko Shiromoto;Dai Haraguchi;Tsuguo Kohama
昆虫不育技术是根据种群生态学和行为生态学原理，广泛应用于抑制或消灭目标害虫种群的技术。SIT的有效性取决于释放的不育雄性与野生雌性交配和授精的能力;然而，使用伽马辐射诱导不育对体细胞和生殖细胞都有负面影响。因此，辐照灭菌会随着时间的推移大大降低交配性能。众所周知，分次剂量辐照（其中灭菌剂量通过一系列较小的辐照输送）可减少辐射损伤。在本研究中，我们评估了分次剂量辐照对Cylas formicarius（Summers）（鞘翅目：Brentidae）生育力，寿命和交配倾向的影响，辐照后16天。 200戈伊的分次剂量辐照诱导完全无菌，无论辐照剂量的数量如何。 尽管在前6天，单次200戈伊剂量（冲绳县SIT项目的现行标准）灭菌的雄性的交配倾向与未辐照象鼻虫的交配倾向相同，但在前12天，连续3次剂量灭菌的雄性的交配倾向至少保持不变。 这些结果表明，分次剂量辐照在C.蚁蛉根除计划。
The sterile insect technique (SIT), based on the principles of population and behavioral ecology, is widely used to suppress or eradicate target pest insect populations. The effectiveness of SIT depends on the ability of released sterile males to mate with and inseminate wild females; however, the use of gamma radiation to induce sterility negatively affects both somatic cells as well as reproductive cells. Consequently, sterilization by irradiation drastically diminishes mating performance over time. It is well known that fractionated‐dose irradiation, in which a sterilizing dose is delivered via a series of smaller irradiations, reduces radiation damage. In the present study, we evaluated the effect of fractionated‐dose irradiation on fertility, longevity, and mating propensity inCylas formicarius(Summers) (Coleoptera: Brentidae) for 16 days after irradiation. Fractionated‐dose irradiation with 200 Gy induced full sterility regardless of the number of radiation doses. Although the mating propensity of males sterilized by a single 200 Gy dose (the current standard of the Okinawa Prefecture SIT program) was equal to that of non‐irradiated weevils for the first 6 days, the mating propensity of males sterilized by a series of three doses was maintained for at least the first 12 days. These results demonstrated that fractionated‐dose irradiation can be highly advantageous inC. formicariuseradication programs.