Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII
Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII
复制标题
用埃及嗜血杆菌的限制性内切酶 R-HaeIII 切割产生的多瘤病毒 DNA 片段的物理图
作者：
J. Summers
用来自埃及嗜血杆菌的限制性内切酶消化多瘤病毒DNA产生至少22个独特片段。已就多瘤基因组上的大小和物理顺序对片段进行了表征，并且已确定了（+）和（-）链的5'至3'方向。采用相邻片段的特异性放射性标记方法确定片段顺序。该技术可用于对复杂DNA消化产生的片段进行排序。
Digestion of polyoma viral DNA with a restriction enzyme from Haemophilus aegyptius generates at least 22 unique fragments. The fragments have been characterized with respect to size and physical order on the polyoma genome, and the 5' to 3' orientation of the (+) and (-) strands has been determined. A method for specific radiolabeling of adjacent fragments was employed to establish the fragment order. This technique may be useful for ordering the fragments produced by digestion of complex DNAs.