DETECTION OF PEMPHIGUS VULGARIS AND PEMPHIGUS FOLIACEUS ANTIGENS BY IMMUNOBLOT ANALYSIS USING DIFFERENT ANTIGEN SOURCES
DETECTION OF PEMPHIGUS VULGARIS AND PEMPHIGUS FOLIACEUS ANTIGENS BY IMMUNOBLOT ANALYSIS USING DIFFERENT ANTIGEN SOURCES
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DOI：
10.1111/1523-1747.ep12874456
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发表时间：
1990-03-01
影响因子：
6.5
通讯作者：
NISHIKAWA, T
中科院分区：
文献类型：
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作者：
HASHIMOTO, T;OGAWA, MM;NISHIKAWA, T
在以人表皮提取物作为抗原来源的免疫印迹分析中，所有 (28/28) 寻常型天疱疮 (Pv) 血清均显示出与 130 kD 蛋白质的特异性反应性。几种（但不是全部）PV 血清与牛口桥粒制剂和培养的人鳞状癌细胞提取物中的类似抗原发生反应。另一方面，在人表皮提取物和牛桥粒制备物中，一些落叶型天疱疮 (Pf) 血清表现出与 150 kD 蛋白质（很可能是桥粒芯糖蛋白 I）的反应性，但没有 Pf 血清与鳞状癌细胞提取物中的该抗原发生反应。此外，4/16 Pv血清还与桥粒制剂中的150-kD蛋白质发生反应，该蛋白质似乎与Pf抗原相同。这些结果表明Pf和Pv两者的抗原与桥粒之间的关系，以及Pv和Pf抗原在抗原分子或表位方面的异质性。此外，这项研究提出了免疫印迹分析可常规用于区分 Pv 和 Pf 抗体的可能性。
In an immunoblot analysis with human epidermal extract as a source of antigens, all (28/28) pemphigus vulgaris (Pv) sera showed a specific reactivity with a 130-kD protein. Several, but not all, Pv sera reacted with similar antigens in both a bovine muzzle desmosome preparation and extract of cultured human squamous carcinoma cells. On the other hand, some pemphigus foliaceus (Pf) sera exhibited reactivity with a 150-kD protein, which is most likely desmoglein I, in both the human epidermal extract and the bovine desmosome preparation, but no Pf serum reacted with this antigen in the squamous carcinoma cell extract. Furthermore, 4/16 Pv sera also reacted with a 150-kD protein in the desmosome preparation, which seemed to be the same as Pf antigen. These results show a relationship between antigens of both Pf and Pv and desmosomes, as well as heterogeneities of both Pv and Pf antigens in terms of antigenic molecules or epitopes. Furthermore, this study presents the possibility that immunoblot analysis can be routinely used for differentiation of Pv and Pf antibodies.