Animal Experimentation: Effect of Essential Amino Acids on Mouse Embryo Viability and Ammonium Production
Animal Experimentation: Effect of Essential Amino Acids on Mouse Embryo Viability and Ammonium Production
复制标题
动物实验：必需氨基酸对小鼠胚胎活力和铵产生的影响
DOI：
10.1023/a:1016657228171
复制
发表时间：
2001
影响因子：
3.1
通讯作者：
D. Gardner
中科院分区：
文献类型：
--
作者：
M. Lane;K. Hooper;D. Gardner
摘要目的：探讨必需氨基酸浓度对小鼠胚胎发育的影响。
AbstractPurpose: To examine the effect of essential amino acids concentrations on mouse embryo development.
Methods: Mouse embryos were cultured in medium with different concentrations of essential amino acids and development to the blastocyst stage and viability assessed. Ammonium production resulting from medium breakdown and amino acid metabolism by embryos were also assessed.
Results: Reducing the essential amino acid concentration significantly increased blastocyst development and cell numbers. Lowering the essential amino acid concentration decreased ammonium production in the medium.
Conclusions: Culture media for the development of preimplantation embryos should have a reduced essential amino acid concentration to facilitate embryo development.