Assessment of the intestinal retention of beta-carotene in humans.
Assessment of the intestinal retention of beta-carotene in humans.
复制标题
评估人类肠道中 β-胡萝卜素的滞留。
DOI：
10.1080/07315724.1994.10718424
复制
发表时间：
1994
影响因子：
3.5
通讯作者：
Frommel,TO
中科院分区：
文献类型：
--
作者：
Shiau,A;Mobarhan,S;Stacewicz-Sapuntzakis,M;Benya,R;Liao,Y;Ford,C;Bowen,P;Friedman,H;Frommel,TO
评估人类对β-胡萝卜素(BC)的肠道吸收以及BC的血浆清除一直是困难的。我们使用全肠道冲洗法(TGWM)来评估BC在通过肠道的转运过程中的保留，以及不同饮食和年龄对BC保留的影响。使用高效液相色谱法来定量年轻和老年受试者的粪便和血清BC浓度，这些受试者在摄入BC或安慰剂之前，无论是否吃饭，都接受了TGWM，以去除所有肠道内容物。膳食含有不同的卡路里和脂肪组合。在没有接受膳食的受试者中，83%的摄入BC是在BC服药后24小时内收集的直肠流出物中回收的。食用者粪便中的含菌量为49-71%。在与任何不同的膳食一起食用BC后，血清中其他类胡萝卜素或维甲酸的浓度没有显著变化。有趣的是，饮食和年龄都影响BC的吸收效率。膳食脂肪含量增加导致年轻受试者服用BC后8小时和24小时内血清BC浓度升高，而热量含量增加导致老年人服用BC后8小时内血清BC浓度下降。结果表明，TGWM为评估BC在人体内的滞留提供了一种准确的手段。
Assessment of the intestinal absorption of beta-carotene (BC) in humans as well as plasma clearance of BC has been difficult. We have used the total gut washout method (TGWM) to assess BC retention during transit through the intestine, as well as the effect that different diets and age have on BC retention.HPLC was used to quantitate fecal and serum BC concentrations from young and elderly subjects who had undergone the TGWM to remove all intestinal contents prior to ingesting BC or placebo with or without a meal. Meals contained different combinations of calories and fat.In subjects receiving no meal, 83% of ingested BC was recovered in rectal effluent collected within 24 hours post-BC administration. The quantity of BC in feces of individuals receiving meals was 49-71%. There was no significant change in serum concentrations of other carotenoids or retinoids following consumption of BC with any of the different meals. Interestingly, both diet and age influenced the efficiency of BC absorption. An increase in dietary fat content resulted in an higher serum BC concentration in young subjects within 8 and at 24 hours post BC administration, whereas a higher caloric content resulted in a decrease in serum BC concentration in older subjects within 8 hours of BC administration.Results indicate that the TGWM provides an accurate means for assessing the intestinal retention of BC in humans.