Daphnane-type diterpenoids from Euphorbia fischeriana Steud and their cytotoxic activities
Daphnane-type diterpenoids from Euphorbia fischeriana Steud and their cytotoxic activities
复制标题
大戟中瑞香型二萜类化合物及其细胞毒活性
DOI：
10.1016/j.fitote.2020.104810
复制
发表时间：
2021-03-01
期刊：
影响因子：
3.4
通讯作者：
Zi, Jiachen
中科院分区：
文献类型：
--
作者：
Xie, Rihan;Xia, Guiyang;Zi, Jiachen
从狼毒大戟（Euphorbia fischeriana Steud）干燥根的提取物中分离得到两种新的瑞香烷型二萜类化合物费氏狼毒素A（1）和费氏狼毒素B（2），以及两种已知的化合物狼毒宁A（3）和狼毒宁A（6）（4）。通过多种光谱方法以及比较其实验和计算的圆二色谱确定了它们的结构，包括绝对立体化学。1和2在其C环中含有一个缩酮基团和一个9,13 - 氧化物桥，这在瑞香烷型二萜类化合物中较为罕见。在细胞毒性试验中，观察到1 - 4对人类癌细胞系（人恶性黑色素瘤细胞系A375、人肝癌细胞系HepG2、人早幼粒细胞白血病细胞系HL - 60、人白种人慢性粒细胞白血病细胞系K562、人宫颈上皮样癌细胞系HeLa）具有中等抑制活性，半数抑制浓度（IC₅₀）值在5.31至21.46 μM之间。
Two new daphnane-type diterpenoids fischerianin A (1) and fischerianin B (2), as well as two known ones langduin A (3) and langduin A(6) (4), were isolated from the extracts of Euphorbia fischeriana Steud dry roots. Their structures including the absolute stereochemistry were determined by various spectroscopic methods and comparing their experimental and calculated CD spectra. 1 and 2 harbor a ketal group and a 9,13-oxide bridge in their C ring which is rare in daphnane-type diterpenoids. In cytotoxic assays, moderately inhibitory activities of 1-4 against human cancer cell lines (human malignant melanoma cell line, A375; human liver carcinoma cell line, HepG2; human promyelocytic leukemia cell line, HL-60; human Caucasian chronic myelogenous leukemia cell line, K562; human cervix epithelioid carcinoma cell line, HeLa) were observed, with IC50 values ranging from 5.31 to 21.46 mu M.