Taichunins E-T, Isopimarane Diterpenes and a 20-nor-Isopimarane, from Aspergillus taichungensis (IBT 19404): Structures and Inhibitory Effects on RANKL-Induced Formation of Multinuclear Osteoclasts
Taichunins E-T, Isopimarane Diterpenes and a 20-nor-Isopimarane, from Aspergillus taichungensis (IBT 19404): Structures and Inhibitory Effects on RANKL-Induced Formation of Multinuclear Osteoclasts
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DOI：
10.1021/acs.jnatprod.1c00486
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发表时间：
2021-08-31
影响因子：
5.1
通讯作者：
Tsukamoto, Sachiko
中科院分区：
文献类型：
--
作者：
El-Desoky, Ahmed H. H.;Inada, Natsumi;Tsukamoto, Sachiko
从台中曲霉（Aspergillus taichungensis）（IBT 19404）中分离得到15个新的异海松烷型二萜化合物taichuninesE-S（1-15）和一个新的20-去甲异海松烷taichuninT（16），以及4个已知化合物。通过核磁共振和质谱确定了这些新化合物的结构，并通过NOESY和TDDFT计算了它们的绝对构型。5 μ M时，Taichunins G、K和N（3、7和10）完全抑制RAW 264细胞中核因子-κ B配体（RANKL）受体激活剂诱导的多核破骨细胞形成，其中3在0.2 μ M浓度时显示92%的抑制。
Fifteen new isopimarane-type diterpenes, taichunins E-S (1-15), and a new 20-nor-isopimarane, taichunin T (16), together with four known compounds were isolated from Aspergillus taichungensis (IBT 19404). The structures of these new compounds were determined by NMR and mass spectroscopy, and their absolute configurations were analyzed by NOESY and TDDFT calculations of ECD spectra. Taichunins G, K, and N (3, 7, and 10) completely inhibited the receptor activator of nuclear factor-kappa B ligand (RANKL)-induced formation of multinuclear osteoclasts in RAW264 cells at 5 mu M, with 3 showing 92% inhibition at a concentration of 0.2 mu M.