Autoimmune Profiling Reveals Peroxiredoxin 6 as a Candidate Traumatic Brain Injury Biomarker.
Autoimmune Profiling Reveals Peroxiredoxin 6 as a Candidate Traumatic Brain Injury Biomarker.
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DOI：
10.1089/neu.2014.3736
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发表时间：
2015-11-15
影响因子：
4.2
通讯作者：
Mueller GP
中科院分区：
文献类型：
--
作者：
Buonora JE;Mousseau M;Jacobowitz DM;Lazarus RC;Yarnell AM;Olsen CH;Pollard HB;Diaz-Arrastia R;Latour L;Mueller GP
大鼠的自身免疫分析显示，抗氧化酶过氧化物还蛋白6 （PRDX6）是创伤性脑损伤（TBI）引起的自身抗体的靶标。与此建议一致，大鼠大脑皮层的免疫组织化学分析表明PRDX6在血管周围空间高度表达，可能包含在星形细胞足突中。因此，开发了一种免疫吸附电化学发光法来研究PRDX6在人体样品中的作用。PRDX6在人血液中可测量，并在人大脑皮层和血小板中高表达。在轻度至中度TBI后4至24小时，循环PRDX6水平比对照组升高了4倍。这些发现表明PRDX6可能作为TBI的生物标志物，自身免疫分析是发现新的TBI生物标志物的可行策略。
Autoimmune profiling in rats revealed the antioxidant enzyme, peroxiredoxin 6 (PRDX6), as a target for autoantibodies evoked in response to traumatic brain injury (TBI). Consistent with this proposal, immunohistochemical analysis of rat cerebral cortex demonstrated that PRDX6 is highly expressed in the perivascular space, presumably contained within astrocytic foot processes. Accordingly, an immunosorbent electrochemiluminescence assay was developed for investigating PRDX6 in human samples. PRDX6 was found to be measurable in human blood and highly expressed in human cerebral cortex and platelets. Circulating levels of PRDX6 were elevated fourfold over control values 4 to 24 h following mild-to-moderate TBI. These findings suggest that PRDX6 may serve as a biomarker for TBI and that autoimmune profiling is a viable strategy for the discovery of novel TBI biomarkers.