First Report of New Delhi Metallo-Beta-Lactamase-1-Producing Klebsiella pneumoniae in Iran
First Report of New Delhi Metallo-Beta-Lactamase-1-Producing Klebsiella pneumoniae in Iran
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DOI：
10.1089/mdr.2012.0078
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发表时间：
2013-02-01
影响因子：
2.6
通讯作者：
Fooladi, Abbas Ali Imani
中科院分区：
文献类型：
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作者：
Shahcheraghi, Fereshteh;Nobari, Saman;Fooladi, Abbas Ali Imani
新德里金属内酰胺酶（NDM-1）是一种新型的金属内酰胺酶（MBL）。不同国家报告了NDM-1阳性菌株的散发病例，这表明存在广泛传播。本研究的目的是检测从德黑兰医院患者分离的肠杆菌科中MBLs。鉴定后，采用Kirby-Bauer法和微量肉汤稀释法进行药敏试验。采用改良霍奇试验（MHT）检测碳青霉烯耐药菌株碳青霉烯酶产量。筛选碳青霉烯耐药菌株的bla（KPC）基因和编码MBLs的基因。美罗培南耐药23株（6.3%），厄他培南耐药11株（3%），亚胺培南耐药4株（1.1%）。11例（47.8%）碳青霉烯耐药菌株MHT阳性。2011年3月，我们检测到一株多重耐药肺炎克雷伯菌分离株，该株对除粘菌素外的所有测试抗生素都具有耐药性。PCR证实该分离物含有bla（NDM-1）、bla（TEM）、bla（SHV）和bla（CTX-M）。这是伊朗首次发现MBL NDM-1的报告。事实证明，ndm -1阳性细菌的迅速传播是治疗和控制传染病的主要挑战。
New Delhi metallo-beta-lactamase (NDM-1) is a novel metallo-beta-lactamase (MBL). Sporadic cases of NDM-1 positive strains have been reported from different countries, suggesting a widespread dissemination. The aim of this study was the detection of MBLs in Enterobacteriaceae isolated from patients in Tehran hospitals. After identification tests, the susceptibility to the antibiotics was done by Kirby-Bauer method and broth microdilution. Carbapenem-resistant isolates were tested for carbapenemase production using the modified Hodge test (MHT). Carbapenem-resistant strains screened for bla(KPC) gene and genes encoding MBLs. Twenty-three isolates (6.3%) were resistant to meropenem, eleven isolates (3%) were resistant to ertapenem, and four isolates (1.1%) were resistant to imipenem. MHT was positive in 11 (47.8%) of the carbapenem-resistant isolates. In March 2011, we detected a multiple drug-resistant Klebsiella pneumoniae isolate that was resistant to all tested antibiotics except colistin. PCR confirmed that this isolate contained bla(NDM-1), bla(TEM), bla(SHV), and bla(CTX-M). This is the first report on the detection of MBL NDM-1 in Iran. The rapid spread of NDM-1-positive bacteria proved to be a major challenge for the treatment and control of infectious diseases.