Comparative analysis of progenitor cells isolated from the iris, pars plana, and ciliary body of the adult porcine eye
Comparative analysis of progenitor cells isolated from the iris, pars plana, and ciliary body of the adult porcine eye
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DOI：
10.1634/stemcells.2007-0035
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发表时间：
2007-01-01
期刊：
影响因子：
5.2
通讯作者：
Ali, Robin R.
中科院分区：
文献类型：
--
作者：
MacNeil, Angus;Pearson, Rachael A.;Ali, Robin R.
在许多视网膜疾病中，光感受器丧失会导致不可逆的失明。合适的供体细胞群的鉴定是相当感兴趣的，因为它们可能用于替代疾病中丢失的光感受器。在大脑的几个区域，包括胚胎视网膜和成人眼睛的睫状上皮中，已经鉴定出产生神经元和神经胶质的干细胞或祖细胞，这提高了自体移植的可能性。然而，很少有系统的研究，以准确的大型哺乳动物成年眼睛的区域产生这样的细胞。在这里，我们首次使用猪眼显示了成人眼睛其他区域中祖细胞的存在，包括睫状体平坦部和虹膜，这些区域在人类中在常规眼科手术中很容易接近。当在生长因子存在下培养时，这些细胞增殖以形成由表达视网膜祖细胞标记物的细胞组成的神经球。使用贴壁单层培养系统，这些细胞可以容易地扩增以使其数量增加超过100万倍并保持祖细胞表型。当生长在基板层粘连蛋白在血清的存在下，来自球体和单层培养的细胞分化成神经元和神经胶质。这些结果表明，来自成年虹膜，睫状体平坦部，和一个大型哺乳动物物种，猪，具有祖细胞特性和神经发生的潜力，从而为移植研究提供了新的供体细胞来源的细胞。
Photoreceptor loss causes irreversible blindness in many retinal diseases. The identification of suitable donor cell populations is of considerable interest because of their potential use to replace the photoreceptors lost in disease. Stem or progenitor cells that give rise to neurons and glia have been identified in several regions of the brain, including the embryonic retina and the ciliary epithelium of the adult eye, raising the possibility of autologous transplantation. However, there has been little systematic investigation into precisely which regions of the large mammalian adult eye give rise to such cells. Here, we show for the first time using the porcine eye the presence of progenitor cells in additional regions of the adult eye, including the pars plana and iris, regions that, in the human, are readily accessible during routine eye surgery. When cultured in the presence of growth factors, these cells proliferate to form neurospheres comprised of cells expressing retinal progenitor markers. Using an adherent monolayer culture system, these cells could be readily expanded to increase their number more than 1 million-fold and maintain a progenitor phenotype. When grown on the substrate laminin in the presence of serum, cells derived from both spheres and monolayer cultures differentiated into neurons and glia. These results suggest that a population of cells derived from the adult iris, pars plana, and ciliary body of a large mammalian species, the pig, has progenitor properties and neurogenic potential, thereby providing novel sources of donor cells for transplantation studies.