Improved in vivo detection of atherosclerotic plaques with a tissue factor-targeting magnetic nanoprobe
Improved in vivo detection of atherosclerotic plaques with a tissue factor-targeting magnetic nanoprobe
复制标题
使用组织因子靶向磁性纳米探针改进动脉粥样硬化斑块的体内检测
DOI：
10.1016/j.actbio.2019.04.014
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发表时间：
2019
影响因子：
9.7
通讯作者：
Hu Yu
中科院分区：
文献类型：
--
作者：
Wei Qiuzhe;Wang Jing;Shi Wei;Zhang Bo;Jiang Huiwen;Du Mengyi;Mei Heng;Hu Yu
动脉粥样硬化斑块破裂可引起急性心脑血管病变。组织因子（TF）是影响动脉粥样硬化斑块发展和血栓形成的关键因子，因此成为动脉粥样硬化斑块检测的潜在靶点。在这项研究中，融合蛋白“增强型绿色荧光蛋白与第一表皮生长因子结构域”（EGFP-EGF 1）和超顺磁性氧化铁纳米粒子（EGFP-EGF 1-SPION）的共轭TF阳性动脉粥样硬化斑块的分子成像进行了探索。在使用磁共振成像检测载脂蛋白E敲除（ApoE−/−）小鼠的动脉粥样硬化斑块中，与普通造影剂相比，EGFP-EGF 1-SPION显示出更高的准确性、上级造影效果和更好的细胞相容性。结论EGFP-EGF 1-SPION是一种很有前景的TF靶向纳米探针，可以准确、特异地检测动脉粥样硬化斑块，为进一步开展心脑血管事件的分子影像学诊断，全面评价动脉粥样硬化提供了新的思路。它仍然具有挑战性的评价斑块特异性和敏感性。本研究构建了一种靶向组织因子的磁性纳米探针，通过磁共振成像技术在体内特异性检测动脉粥样硬化斑块，为进一步完善动脉粥样硬化斑块的诊断技术，为动脉粥样硬化的治疗提供分子水平的指导。该策略对心脑血管事件的预防、诊断和疗效评价具有重要的临床意义。
Rupture of atherosclerotic plaques causes acute cardiovascular and cerebrovascular pathology. Tissue factor (TF) is a key factor that affects the development of atherosclerotic plaques and the formation of thrombus and thus constitutes a potential target for the detection of atherosclerotic plaques. In this study, the conjugation of the fusion protein ‘enhanced green fluorescent protein with the first epidermal growth factor domain’ (EGFP-EGF1) and superparamagnetic iron oxide nanoparticles (EGFP-EGF1-SPIONs) was explored for molecular imaging of TF-positive atherosclerotic plaques. EGFP-EGF1-SPIONs showed improved accuracy, superior contrast effects, and better cytocompatibility compared with common contrast agents in the detection of atherosclerotic plaques of apolipoprotein E knockout (ApoE−/−) mice using magnetic resonance imaging. In conclusion, EGFP-EGF1-SPION is a promising TF-targeting nanoprobe to precisely and specifically detect atherosclerotic plaques, which may improve molecular imaging diagnosis of cardiovascular and cerebrovascular events for the comprehensive evaluation of atherosclerosis.Statement of SignificanceTraditional methods can only display the status of atherosclerosis, but not forecast the progress of lesions efficiently. It remains challenging to evaluate the plaques specifically and sensitively. In this study, we constructed a tissue factor-targeted magnetic nanoprobe to specifically detect plaques by magnetic resonance imagingin vivo, which will improve the diagnostic technology for atherosclerotic plaques and offer molecular level guidance to treat atherosclerosis. Furthermore, this strategy has critical clinical significance on prevention, diagnosis and therapeutic evaluation of cardio-cerebral vascular events.