Molecular cloning and characterization of a novel retinoic acid-inducible gene that encodes a putative G protein-coupled receptor
Molecular cloning and characterization of a novel retinoic acid-inducible gene that encodes a putative G protein-coupled receptor
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DOI：
10.1074/jbc.273.52.35008
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发表时间：
1998-12-25
影响因子：
4.8
通讯作者：
Lotan, R
中科院分区：
文献类型：
--
作者：
Cheng, YJ;Lotan, R
类维生素A如全反式维甲酸（ATRA）对细胞生长、分化和凋亡的作用被认为是由核类维生素A受体介导的，其参与靶基因的配体依赖性转录激活。利用差异显示技术，我们在人鳞状细胞癌细胞系USCC-22 B中发现了一个新的基因，命名为维甲酸诱导基因1（RAIG 1）。RAIG 1的两个转录本分别为2.4和6.8个酶对，具有相同的ORF，编码一个357个氨基酸的多肽。RAIG 1 mRNA在胎儿和成人肺组织中高水平表达。ATRA诱导RAIG 1表达是快速的（2小时内），在1 nM至1 μ M的范围内呈剂量依赖性。组成RAIG 1 mRNA水平，这是低的5个头颈部和6个肺癌细胞系中的4个，增加ATRA治疗后，在大多数细胞系。RAIG 1蛋白含有7个跨膜结构域，具有G蛋白偶联受体的特征。RAIG 1与绿色荧光蛋白的融合蛋白定位于细胞表面膜和核周囊泡中。RAIG 1定位于染色体12p12.3-p13，为维甲酸和G蛋白信号通路之间可能的相互作用提供了新的证据。
The effects of retinoids such as all-trans-retinoic acid (ATRA) on cell growth, differentiation, and apoptosis are thought to be mediated by nuclear retinoid receptors, which are involved in ligand-dependent transcriptional activation of target genes. Using differential display, we identified the cDNA of a novel gene, designated retinoic acid-inducible gene 1 (RAIG1), which was induced by ATRA in the squamous carcinoma cell line UMSCC-22B, Two RAIG1 transcripts of 2.4 and 6.8 kilobase pairs, respectively, have the same ORF that encodes a 357-amino acid polypeptide. RAIG1 mRNA is expressed at high level in fetal and adult lung tissues. Induction of RAIG1 expression by ATRA is rapid (within 2 h) and dose-dependent in the range between 1 nM to 1 mu M. The constitutive RAIG1 mRNA levels, which were low in three of five head and neck and four of six lung cancer cell lines, increased after ATRA treatment in most cell lines. The deduced RAIG1 protein sequence contains seven transmembrane domains, characteristic of G protein-coupled receptors, A fusion protein of RAIG1 and the green fluorescent protein was localized in the cell surface membrane and perinuclear vesicles in transiently transfected cells. RAIG1 was mapped to chromosome 12p12.3-p13, Our results provide novel evidence for a possible interaction between retinoid and G protein signaling pathways.