Kordiimonas sediminis sp nov., isolated from a sea cucumber culture pond
Kordiimonas sediminis sp nov., isolated from a sea cucumber culture pond
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Kordiimonas sediminis sp nov.，从海参养殖池中分离出来
DOI：
10.1007/s10482-016-0671-z
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发表时间：
2016
影响因子：
2.6
通讯作者：
Du Zong-Jun
中科院分区：
文献类型：
--
作者：
Zhang Heng-Xi;Zhao Jin-Xin;Chen Guan-Jun;Du Zong-Jun
从威海市海参养殖池的底泥中分离到一株海洋细菌，命名为N39 T。N39 T菌株的细胞为革兰氏染色阴性，厌氧性，有活动的杆状细胞，过氧化氢酶和氧化酶反应阴性。发现菌株N39 T在pH 8.0-8.5、35-37 °C和在约3.0%（w/v）NaCl存在下最佳生长。16 S rRNA基因序列分析表明，N39 T菌株属于科尔迪单胞菌科（Kordiimonadaceae）的科尔迪单胞菌属（Kordiimonas），与Kordiimonas lacusJCM 16261 T（95.9%）、Kordiimonas aquimonas MEBiC 06554 T（95.1%）、Kordiimonas gwangyangensis JCM 12864 T（94.2%）和Kordiimonas aestuarii 101 - 1 T（93.8%）的亲缘关系较近。泛醌10（Q-10）被认为是主要的呼吸醌。细胞内脂肪酸主要为iso-C17：1ω 9 c、iso-C17：0、iso-C15：0和C17：1ω6c。发现主要极性脂质为磷脂酰甘油、磷脂酰乙醇胺和二磷脂酰甘油。菌株N39 T的DNA G + C含量为50.8%。根据基因型、化学分类学和表型数据，菌株N39 T被认为是科氏单胞菌属中的一个新种，命名为Kordiimonasediminissp.十一是建议。模式菌株为N39 T（=KCTC 42590 T = MCCC 1H 00112 T）。
A marine bacterium, designated strain N39T, was isolated from a sediment sample collected at a sea cucumber culture pond in Weihai, China. Cells of strain N39Twere observed to be Gram-stain negative, facultatively anaerobic, motile rods showing catalase and oxidase negative reactions. Strain N39Twas found to grow optimally at pH 8.0–8.5, 35–37 °C and in the presence of approximately 3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain N39Tbelongs to the genusKordiimonasin the familyKordiimonadaceae, appearing closely related toKordiimonas lacusJCM 16261T(95.9 %),Kordiimonas aquimarisMEBiC06554T(95.1 %),Kordiimonas gwangyangensisJCM 12864T(94.2 %) andKordiimonas aestuarii101-1T(93.8 %). Ubiquinone 10 (Q-10) was found to be the major respiratory quinone. The dominant cellular fatty acids were identified as iso-C17:1ω9c, iso-C17:0,iso-C15:0and C17:1ω6c. The predominant polar lipids were found to be phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G + C content of strain N39Tis 50.8 %. On the basis of genotypic, chemotaxonomic and phenotypic data, strain N39Tis concluded to represent a novel species within the genusKordiimonas, for which the nameKordiimonas sediminissp. nov. is proposed. The type strain is N39T(=KCTC 42590T= MCCC 1H00112T).