Sindbis virus: propagation, quantification, and storage.
Sindbis virus: propagation, quantification, and storage.
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DOI：
10.1002/9780471729259.mc15b01s16
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发表时间：
2010-02-01
影响因子：
--
通讯作者：
Brown, Dennis T
中科院分区：
文献类型：
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作者：
Hernandez, Raquel;Sinodis, Christine;Brown, Dennis T
甲病毒的原型辛德毕斯病毒具有广泛的宿主范围。在自然界中，辛德毕斯病毒在一个复杂的传播循环中从昆虫载体穿梭到脊椎动物宿主，再回到昆虫载体。因此，辛德毕斯病毒必须能够在两种生物化学和遗传上不同的宿主（无脊椎动物和脊椎动物）中复制。在实验室中，辛德毕斯在脊椎动物和无脊椎动物来源的各种培养细胞中生长至高滴度。辛德毕斯病毒在几种哺乳动物细胞系以及某些蚊子细胞上的感染性很容易滴定。可以获得几种辛德毕斯病毒株的全长cDNA克隆，从中可以合成感染性RNA，从而使病毒的遗传操作成为可能。通过电穿孔转染哺乳动物和昆虫细胞促进了RNA突变体在所选细胞系中的表达。
The prototype of the Alphaviruses, Sindbis virus, has a broad host range. In nature, Sindbis virus shuttles from an insect vector to a vertebrate host and back to the insect vector in a complex transmission cycle. Sindbis virus must, therefore, be able to replicate in two biochemically and genetically divergent hosts, invertebrates and vertebrates. In the laboratory, Sindbis grows to high titers in a large variety of cultured cells of both vertebrate and invertebrate origin. Sindbis virus is easily titered for infectivity on several mammalian cell lines as well as certain mosquito cells. Full-length cDNA clones of several strains of Sindbis virus are available from which infectious RNA can be synthesized, making possible the genetic manipulation of the virus. Transfection of mammalian and insect cells by electroporation has facilitated expression of RNA mutants in the cell lines of choice.