CELLULAR-LOCALIZATION OF HUMAN IMMUNODEFICIENCY VIRUS-INFECTION WITHIN THE BRAINS OF ACQUIRED-IMMUNE-DEFICIENCY-SYNDROME PATIENTS
CELLULAR-LOCALIZATION OF HUMAN IMMUNODEFICIENCY VIRUS-INFECTION WITHIN THE BRAINS OF ACQUIRED-IMMUNE-DEFICIENCY-SYNDROME PATIENTS
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DOI：
10.1073/pnas.83.18.7089
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发表时间：
1986-09-01
影响因子：
11.1
通讯作者：
OLDSTONE, MBA
中科院分区：
文献类型：
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作者：
WILEY, CA;SCHRIER, RD;OLDSTONE, MBA
中枢神经系统（CNS）功能障碍是获得性免疫缺陷综合征（AIDS）的一个显著特征。这些患者中的许多人患有与中枢神经系统病毒感染一致的亚急性脑炎。我们研究了12名艾滋病患者的大脑，使用原位杂交技术鉴定人类免疫缺陷病毒[HIV，被其他人称为人类t细胞嗜淋巴病毒III型（HTLV-III），淋巴结病相关病毒（LAV），艾滋病相关逆转录病毒（ARV）]的核酸序列和免疫细胞化学鉴定病毒和细胞蛋白。9例患者中枢神经系统有明显的HIV感染。在所有被检查的大脑中，白质比灰质更严重。大多数病例的感染局限于毛细血管内皮细胞、单核炎症细胞和巨细胞。在一例中枢神经系统严重受累的病例中，在一些星形胶质细胞和神经元中可见低水平感染。这些结果表明中枢神经系统功能障碍是由于间接影响而不是神经元或神经胶质感染。
Dysfunction of the central nervous system (CNS) is a prominent feature of the acquired immune deficiency syndrome (AIDS). Many of these patients have a subacute encephalitis consistent with a viral infection of the CNS. We studied the brains of 12 AIDS patients using in situ hybridization to identify human immunodeficiency virus [HIV, referred to by others as human T-cell lymphotropic virus type III (HTLV-III), lymphadenopathy-associated virus (LAV), AIDS-associated retrovirus (ARV)] nucleic acid sequences and immunocytochemistry to identify viral and cellular proteins. Nine patients had significant HIV infection in the CNS. In all examined brains, the white matter was more severely involved than the grey matter. In most cases the infection was restricted to capillary endothelial cells, mononuclear inflammatory cells, and giant cells. In a single case with severe CNS involvement, a low-level infection was seen in some astrocytes and neurons. These results suggest that CNS dysfunction is due to indirect effect rather than neuronal or glial infection.