Rearrangement-enhancing element upstream of the mouse immunoglobulin kappa chain J cluster
Rearrangement-enhancing element upstream of the mouse immunoglobulin kappa chain J cluster
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DOI：
10.1126/science.271.5254.1416
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发表时间：
1996-03-08
期刊：
影响因子：
56.9
通讯作者：
Weill, JC
中科院分区：
文献类型：
--
作者：
Ferradini, L;Gu, H;Weill, JC
转录调控元件已被证明对于小鼠前体 B 细胞中免疫球蛋白基因重排的发育调控是必要的，但还不够。在鸡 lambda 轻链基因座中，V-J 间插序列中的其他元件参与重排的负向和正向调节。在这里，位于 J(kappa) 分散器上游的 V-kappa-J(kappa) 干预序列中的鸡元件的小鼠同源物的突变被证明可显着减少重排。这种顺式作用重组增强元件影响重排过程，但不参与调节转录。
Transcriptional regulatory elements have been shown to be necessary but not sufficient for the developmental regulation of immunoglobulin gene rearrangement in mouse precursor B cells. In the chicken lambda light chain locus, additional elements in the V-J intervening sequence are involved in negative and positive regulation of rearrangement. Here, mutation of the mouse homolog of a chicken element, located in the V-kappa-J(kappa) intervening sequence upstream of the J(kappa) duster, was shown to significantly decrease rearrangement. This cis-acting recombination-enhancing element affects the rearrangement process without being involved in regulating transcription.