Protistan community analysis: key findings of a large-scale molecular sampling.
Protistan community analysis: key findings of a large-scale molecular sampling.
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DOI：
10.1038/ismej.2016.10
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发表时间：
2016-09
期刊：
影响因子：
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通讯作者：
Boenigk J
中科院分区：
文献类型：
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作者：
Grossmann L;Jensen M;Heider D;Jost S;Glücksman E;Hartikainen H;Mahamdallie SS;Gardner M;Hoffmann D;Bass D;Boenigk J
原生生物可能是微生物生命形式中最丰富的谱系，但在很大程度上仍然未知。高通量测序技术提供了深入筛选整个生境的机会，并能够对不同生境进行详细比较，以测量、比较和绘制原生生物多样性。这种比较往往受到单一研究中样本数量少和研究之间缺乏标准化的限制。在这里，我们使用标准化的PCR协议和生物信息学管道分析了来自10个采样活动的232个样本。我们表明，原生生物群落模式是高度一致的栖息地类型和地理区域内，样品处理标准化。社区配置文件只有微弱的影响，最丰富的类群的丰度波动，因此，提供了一个良好的基础，超越随机短期波动的社区组成的栖息地比较。此外，我们提供的证据表明，分布模式不仅仅是随机过程的结果。不同的栖息地类型和不同的分类群占主导地位的类群具有不同的分布模式，反映了他们的生态与扩散和栖息地殖民。然而，没有系统的转移的分布格局与分类单元的丰度。
Protists are perhaps the most lineage-rich of microbial lifeforms, but remain largely unknown. High-throughput sequencing technologies provide opportunities to screen whole habitats in depth and enable detailed comparisons of different habitats to measure, compare and map protistan diversity. Such comparisons are often limited by low sample numbers within single studies and a lack of standardisation between studies. Here, we analysed 232 samples from 10 sampling campaigns using a standardised PCR protocol and bioinformatics pipeline. We show that protistan community patterns are highly consistent within habitat types and geographic regions, provided that sample processing is standardised. Community profiles are only weakly affected by fluctuations of the abundances of the most abundant taxa and, therefore, provide a sound basis for habitat comparison beyond random short-term fluctuations in the community composition. Further, we provide evidence that distribution patterns are not solely resulting from random processes. Distinct habitat types and distinct taxonomic groups are dominated by taxa with distinct distribution patterns that reflect their ecology with respect to dispersal and habitat colonisation. However, there is no systematic shift of the distribution pattern with taxon abundance.