ECM molecules mediate both Schwann cell proliferation and activation to enhance neurite outgrowth
ECM molecules mediate both Schwann cell proliferation and activation to enhance neurite outgrowth
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DOI：
10.1089/ten.2007.0055
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发表时间：
2007-12-01
影响因子：
--
通讯作者：
Kingham, Paul J.
中科院分区：
文献类型：
--
作者：
Armstrong, Stephanie J.;Wiberg, Mikael;Kingham, Paul J.
利用生物材料和细胞相结合的组织工程代表了神经修复的新方法。我们研究了细胞外基质（ECM）分子对雪旺细胞（SC）在神经导管材料聚-3-羟基丁酸酯（PHB）上附着和增殖的影响，以及SC对体外神经突起生长的影响。初始SC附着到PHB，垫不受ECM分子的影响，但增殖增加（层粘连蛋白>纤连蛋白>胶原蛋白）。将SC接种到悬浮在NG 108 -15细胞单层上方的ECM包被的培养插入物上，确定释放的扩散因子的作用。还研究了两种细胞类型之间的直接接触对ECM分子的影响。在这两种系统中，SC增加了每个细胞的神经突数量和NG 108 -15细胞出芽神经突的百分比。NG 108 -15细胞生长在直接接触的SC有显着较长的神经突起比那些暴露于扩散因子时，接种在层粘连蛋白或纤连蛋白。从在ECM分子上培养的SC释放的扩散因子似乎启动神经突生长，而SC-神经元接触促进神经突伸长。SC增殖是最大的聚-D-赖氨酸包被的表面，但这些细胞并没有影响神经突起生长的层粘连蛋白或纤连蛋白的水平。这表明ECM分子增加细胞数量并激活SC以释放轴突促进因子。将ECM分子添加到含有SC的PHB神经导管中可能为神经损伤的治疗提供益处。
Tissue engineering using a combination of biomaterials and cells represents a new approach to nerve repair. We have investigated the effect that extracellular matrix (ECM) molecules have on Schwann cell (SC) attachment and proliferation on the nerve conduit material poly-3-hydroxybutyrate (PHB), and SC influence on neurite outgrowth in vitro. Initial SC attachment to PHB, mats was unaffected by ECM molecules but proliferation increased (laminin > fibronectin > collagen). SCs seeded onto ECM-coated culture inserts suspended above a monolayer of NG108-15 cells determined the effect of released diffusible factors. The effect of direct contact between the two cell types on ECM molecules was also investigated. In both systems SCs enhanced neurite number per cell and percentage of NG108-15 cells sprouting neurites. NG108-15 cells grown in direct contact with SCs had significantly longer neurites than those exposed to diffusible factors when seeded on laminin or fibronectin. Diffusible factors released from SCs cultured on ECM molecules appear to initiate neurite outgrowth, whereas SC-neuron contact promotes neurite elongation. SC proliferation was maximal on poly-D-lysine-coated surfaces, but these cells did not influence neurite outgrowth to the levels of laminin or fibronectin. This suggests that ECM molecules enhance cell number and activate SCs to release neurite promoting factors. Addition of ECM molecules to PHB nerve conduits containing SCs is likely to provide benefits for the treatment of nerve injuries.