Quantitative analysis of the dendritic morphology of corticocortical projection neurons in the macaque monkey association cortex
Quantitative analysis of the dendritic morphology of corticocortical projection neurons in the macaque monkey association cortex
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DOI：
10.1016/s0306-4522(02)00305-6
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Hof, PR
中科院分区：
文献类型：
--
作者：
Duan, H;Wearne, L;Hof, PR
灵长类动物大脑皮层的多模态关联区域相互连接，在认知中起着至关重要的作用。非人类灵长类动物前额叶皮层的第46区接收来自几个关联区域的直接输入，其中包括位于上级颞沟的皮层区域。我们研究了是否投射神经元提供这样的皮质投射不同的树突状形态从锥体神经元投射局部区域内46。在年轻的猕猴体内逆行运输的皮质皮质投射神经元的特定集进行了鉴定。通过靶向细胞内注射荧光黄，在脑切片中观察逆行标记神经元的完整树突，并使用计算机辅助形态计量学进行三维重建。在形成长投射（从上级颞叶皮层到前额区46）以及局部投射（在区域46内）的第III层锥体神经元中分析总树突长度、节段数、棘数和棘密度。Sholl分析也被用来比较这两组神经元的复杂性。我们的研究结果表明，连接颞叶和前额皮质的皮质皮质投射神经元比在区域46内局部投射的锥体神经元具有更长、更复杂的树突状乔木和更多的棘。这些神经元更复杂的树突分支可能与它们参与皮层网络有关，这些网络需要在细胞水平上广泛会聚多个传入神经。(C)2002年IBRO。由Elsevier Science Ltd.出版，版权所有。
The polymodal association areas of the primate cerebral cortex are heavily interconnected and play a crucial role in cognition. Area 46 of the prefrontal cortex in non-human primates receives direct inputs from several association areas, among them the cortical regions lining the superior temporal sulcus. We examined whether projection neurons providing such a corticocortical projection differ in their dendritic morphology from pyramidal neurons projecting locally within area 46. Specific sets of corticocortical projection neurons were identified by in vivo retrograde transport in young macaque monkeys. Full dendritic arbors of retrogradely labeled neurons were visualized in brain slices by targeted intracellular injection of Lucifer Yellow, and reconstructed three-dimension ally using computer-assisted morphometty. Total dendritic length, numbers of segments, numbers of spines, and spine density were analyzed in layer III pyramidal neurons forming long projections (from the superior temporal cortex to prefrontal area 46), as well as local projections (within area 46). Sholl analysis was also used to compare the complexity of these two groups of neurons. Our results demonstrate that [on., corticocortical projection neurons connecting the temporal and prefrontal cortex have longer, more complex dendritic arbors and more spines than pyramidal neurons projecting locally within area 46. The more complex dendritic arborization of such neurons is likely linked to their participation in cortical networks that require extensive convergence of multiple afferents at the cellular level. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.