Vacuolization of HeLa cells by a partially purified Clostridium histolyticum cytotoxin.
Vacuolization of HeLa cells by a partially purified Clostridium histolyticum cytotoxin.
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部分纯化的溶组织梭菌细胞毒素使 HeLa 细胞空泡化。
DOI：
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发表时间：
2006
影响因子：
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通讯作者：
G. Martirosian
中科院分区：
文献类型：
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作者：
E. Jankowska;J. Jóźwiak;T. Grzela;A. Komar;J. Niderla;M. Lazarczyk;G. Korczak;S. Moskalewski;G. Martirosian
采用硫酸铵沉淀法、凝胶过滤法和疏水相互作用色谱法对溶组织梭菌液泡细胞毒素进行了部分纯化。2小时后，光镜下可见HeLa细胞空泡化，8小时后可见空泡化。透射电镜显示存在大量空泡，线粒体基质凝结，细胞质密度增加，异染色质数量增加。无论是电子显微镜还是用荧光素标记的膜联蛋白V和碘化丙啶进行的细胞凋亡/坏死鉴别试验，还是DNA片段化试验，均未检测到细胞凋亡。用Fluo-4 AM标记细胞后，流式细胞术检测钙离子内流。巴菲霉素A1抑制了溶血c细胞毒素对HeLa细胞的空泡形成，提示H+ - atp酶参与了空泡的形成。
Clostridium histolyticum vacuolating cytotoxin was partially purified from culture broth using ammonium sulfate precipitation, gel filtration and hydrophobic interaction chromatography. The toxin caused vacuolization of HeLa cells visible under a light microscope after 2 h and distinct after 8 h. Transmission electron microscopy revealed the presence of numerous vacuoles, condensation of the mitochondrial matrix, increased cytoplasm density and increased amounts of heterochromatin. Apoptosis was not detected either by electron microscopy or by an apoptosis/necrosis discrimination assay with fluorescein-labeled annexin V and propidium iodide, or DNA fragmentation assay. Calcium ion influx was detected by flow cytometry after labeling cells with Fluo-4 AM. Vacuolation of HeLa cells by C. histolyticum cytotoxin was inhibited by bafilomycin A1, suggesting involvement of H+ -ATPase in the formation of vacuoles.
DOI：
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发表时间：
1992
期刊：
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影响因子：
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作者：
Mookhtiar Ka;V. He
通讯作者：
Mookhtiar Ka;V. He
艰难梭菌毒素 B 会激活细胞毒性过程中肌动蛋白分解所需的钙流入。
DOI：
10.1152/ajpgi.1995.268.3.g487
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
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作者：
Gilbert,RJ;Pothoulakis,C;LaMont,JT;Yakubovich,M
通讯作者：
Yakubovich,M