A surprisingly large RNase P RNA in Candida glabrata
A surprisingly large RNase P RNA in Candida glabrata
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作者：
Kachouri, R;Stribinskis, S;Li, Y
我们在人类病原体念珠菌光秃酵母中发现了一个极大的核糖核酸酶P (RNase P) RNA (RPR1)，并证实该分子在这种半真菌酵母的活性酶复合体中有表达和存在。C. glabrata序列与其他36种半真菌RNase P rna（缩写为P rna）的结构比对使我们能够表征插入类型。此外，在最近测序的基因组中检索到15个新的P RNA序列，包括白色念珠菌、光面念珠菌、汉氏德巴酵母、棉棉酵母、乳酸克卢维菌、瓦氏克卢维菌、castellia Naumovia、kudriavzevii酵母菌、mikatae酵母菌和多脂耶氏耶氏菌；并通过PCR扩增其他念珠菌种类（guilliermondii、krusei念珠菌、parapsilosis念珠菌、stellatoidea念珠菌和热带念珠菌）。系统发育比较分析确定了半囊菌二级结构共识，该共识在所有物种中都具有可变插入或缺失的保守核心。最显著的变异是在C glabrata P RNA中发现的，其中特异性区域存在三个超过700 nt的插入。这种P RNA的长度是已知的其他同源P RNA的两倍多，是最小P RNA的八倍大。因此，RNase P RNA在大小变化和结构多样性方面代表了最多样化的非编码RNA之一。
We have found an extremely large ribonuclease P (RNase P) RNA (RPR1) in the human pathogen Candida glabrata and verified that this molecule is expressed and present in the active enzyme complex of this hemiascomycete yeast. A structural alignment of the C. glabrata sequence with 36 other hemiascomycete RNase P RNAs (abbreviated as P RNAs) allows us to characterize the types of insertions. In addition, 15 P RNA sequences were newly characterized by searching in the recently sequenced genomes Candida albicans, C. glabrata, Debaryomyces hansenii, Eremothecium gossypii, Kluyveromyces lactis, Kluyveromyces waltii, Naumovia castellii, Saccharomyces kudriavzevii, Saccharomyces mikatae, and Yarrowia lipolytica; and by PCR amplification for other Candida species (Candida guilliermondii, Candida krusei, Candida parapsilosis, Candida stellatoidea, and Candida tropicalis). The phylogenetic comparative analysis identifies a hemiascomycete secondary structure consensus that presents a conserved core in all species with variable insertions or deletions. The most significant variability is found in C glabrata P RNA in which three insertions exceeding in total 700 nt are present in the Specificity domain. This P RNA is more than twice the length of any other homologous P RNAs known in the three domains of life and is eight times the size of the smallest. RNase P RNA, therefore, represents one of the most diversified noncoding RNAs in terms of size variation and structural diversity.