USE OF MONOCLONAL-ANTIBODIES TO ANALYZE PERIPHERAL-BLOOD AND SALIVARY-GLAND LYMPHOCYTE SUBSETS IN SJOGRENS SYNDROME
USE OF MONOCLONAL-ANTIBODIES TO ANALYZE PERIPHERAL-BLOOD AND SALIVARY-GLAND LYMPHOCYTE SUBSETS IN SJOGRENS SYNDROME
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DOI：
10.1002/art.1780250410
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发表时间：
1982-01-01
影响因子：
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通讯作者：
VAUGHAN, JH
中科院分区：
文献类型：
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作者：
FOX, RI;CARSTENS, SA;VAUGHAN, JH
应用抗细胞表面抗原的单克隆抗体，对15例原发性干燥综合征患者的淋巴细胞亚群进行了研究。OKT 8阳性细胞（与T抑制细胞/细胞毒性细胞反应）的绝对数在这些患者中显著减少（353 ± 0.001）。186/mm 3）与年龄匹配的对照组（631 . ±. 150/mm ~ 3）（P < 0.001）。两组中OKT 4阳性细胞（与T辅助/诱导细胞反应）的数量相当（932 ± 0.001）。588/mm3 vs. 1.073 . ±. 290/mm3）。在这些患者中，67%的患者的OKT 4/OKT 8反应性外周血淋巴细胞的比率增加（> 2.4），范围为1.0至6.4（正常= 1.8 ± 0.1）。0.3）。OKT 4阳性细胞是唇活检标本中的主要亚群，用免疫荧光或免疫过氧化物酶技术染色;在所有5例患者中，OKT 4/OKT 8比值超过3.0。在1例假性淋巴瘤患者中，淋巴结活检标本含有80%的T细胞，OKT 4/OKT 8比值为3.2。因此，OKT 4阳性细胞在外周血淋巴细胞以及原发性干燥综合征的炎症部位中占主导地位。原发性干燥综合征中OKT 8阳性细胞数量的减少可能不是由循环自身抗体引起的，因为患者的血清不与正常的OKT 8阳性细胞反应。使用美洲商陆有丝分裂原的功能研究表明，正常和患者外周血淋巴细胞中的OKT 4阳性亚群中含有合成IG的T辅助细胞活性。通过补体介导的裂解去除OKT 8阳性细胞并不导致类风湿因子的IG合成或产生增加。通过使用单克隆抗体鉴定外周血淋巴细胞亚群以及这些亚群与组织浸润和自身抗体产生的关系，为进一步了解原发性干燥综合征的发病机制提供了依据。
Using [mouse] monoclonal antibodies to cell surface antigens, lymphocyte subsets in 15 patients with primary Sjogren''s syndrome were studied. The absolute number of OKT8-positive cells (reactive with T suppressor/cytotoxic cells) was significantly decreased in such patients (353 .+-. 186/mm3) compared to age-matched controls (631 .+-. 150/mm3) (P < 0.001). The number of OKT4-positive cells (reactive with T helper/inducer cells) was comparable in both groups (932 .+-. 588/mm3 vs. 1.073 .+-. 290/mm3). The ratio of OKT4/OKT8-reactive peripheral blood lymphocytes was increased (> 2.4) in 67% of these patients and ranged from 1.0 to 6.4 (normal = 1.8 .+-. 0.3). OKT4-positive cells were the predominant subset in lip biopsy specimens stained with immunofluorescence or immunoperoxidase techniques; the OKT4/OKT8 ratio exceeded 3.0 in all 5 patients examined. In 1 patient with pseudolymphoma, a lymph node biopsy specimen contained 80% T cells with an OKT4/OKT8 ratio of 3.2. Thus, OKT4-positive cells predominated in the peripheral blood lymphocytes as well as in sites of inflammation in primary Sjogren''s syndrome. The decreased number of OKT8-positive cells in primary Sjogren''s syndrome was probably not caused by circulating autoantibody, since patients'' sera did not react with normal OKT8-positive cells. Functional studies using pokeweed mitogen demonstrated that T helper cell activity for Ig synthesis was contained in the OKT4-positive subset in both normal and patients'' peripheral blood lymphocytes. Removal of OKT8-positive cells by complement-mediated lysis did not lead to increased Ig synthesis or production of rheumatoid factor. The identification of peripheral blood lymphocyte subsets by use of monoclonal antibodies and the relationship of these subsets to tissue infiltrates and autoantibody production provide further insight into the pathogenesis of primary Sjogren''s syndrome.