Effect of the conceptus on quantitative and qualitative aspects of uterine secretion in pigs.
Effect of the conceptus on quantitative and qualitative aspects of uterine secretion in pigs.
复制标题
受孕对猪子宫分泌物定量和定性的影响。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
R. Roberts
中科院分区：
文献类型：
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作者：
S. Basha;F. Bazer;R. Roberts
取妊娠和假孕动物子宫内膜(第11~15天戊酸雌二醇5 mg)60天，单侧妊娠猪60天的妊娠和非妊娠的角，发情周期12天的非妊娠猪的角。组织在L-[34S]蛋氨酸存在下培养24 h，组织和培养液分别进行双向聚丙烯酰胺凝胶电泳法分析。干凝胶放射自显影鉴定放射性多肽。除周期动物外，所有动物的组织都向培养液中释放了一组相同的多肽：主要放射性产物是4种低相对分子质量的酸性多肽和几种碱性蛋白，包括紫色磷酸酶、子宫铁蛋白和溶菌酶。在单独的实验中，将3头单侧妊娠猪的外植体用L-[~3H]亮氨酸培养，在新鲜重量的基础上，非妊娠角组织在24小时内向培养液中释放的放射性大分子物质明显少于妊娠角组织。因此，似乎虽然怀孕子宫分泌的本质是母体荷尔蒙调节的结果，但从数量上讲，支持妊娠的组织比子宫空闲区域的组织更活跃。
Endometrial explants were removed from uteri of animals pregnant and pseudopregnant (5 mg oestradiol valerate on Days 11--15) for 60 days, from the gravid and non-gravid horns of unilaterally pregnant pigs at Day 60 of pregnancy and fron non-pregnant animals at Day 12 of the oestrous cycle. The tissues were cultured in the presence of L-[34S]methionine for 24 h, and the tissues and medium were then analysed separately by two-dimensional polyacrylamide gel electrophoresis. Radioactive polypeptides were identified by autoradiography of dried gels. Tissues from all all except the cyclic animals released an identical group of polypeptides into the culture medium: the major radioactive products were 4 acidic polypeptides of low molecular weight and several basic proteins, which included the purple phosphatase uteroferrin, and lysozyme. In separate experiments explants from 3 unilaterally pregnant pigs were cultured with L-[3H]leucine, and, on a fresh weight basis, the tissue from the non-gravid horn released significantly less radioactive macromolecular material into the medium in 24 h than did tissue from gravid horns. It therefore appears that although the nature of the secretion produced by the pregnant uterus is a consequence of maternal hormonal regulation alone, the tissue underlying a conceptus is quantitatively more active than that from unoccupied regions of a uterus.