Amperometric lactate oxidase catheter for real-time lactate monitoring based on thin film technology.
Amperometric lactate oxidase catheter for real-time lactate monitoring based on thin film technology.
复制标题
DOI：
10.1016/s0956-5663(97)00014-6
复制
发表时间：
1997
影响因子：
12.6
通讯作者：
D. Pfeiffer;B. Möller;N. Klimes;J. Szeponik;S. Fischer
中科院分区：
文献类型：
--
作者：
D. Pfeiffer;B. Möller;N. Klimes;J. Szeponik;S. Fischer
一种安培法乳酸氧化酶导管已被开发用于体内实时乳酸监测。 1 × 3 mm Pt-Ag/AgCl 薄膜电极的电化学行为不受乳酸氧化酶-聚氨酯覆盖的显着影响。伽马射线 (25 kGy) 适用于灭菌程序。最终的乳酸导管的特点是线性浓度范围在 0·5 和 20 mmol/l 乳酸之间，灵敏度约为 2 nA mmol−11−1 乳酸。准确性通过对照血清的测量来证明。生理和病理材料均与声明值相关良好。干燥储存的乳酸导管需要大约10分钟进行水合，其特点是响应时间t98%小于2分钟。使用乳酸导管进行的离体全血测量 (y) 与 BIOSEN Med L (x) 的相关性为 y = (1·010x + 0·513) mmol/l (r = 0·9748)。离体连续导管操作获得的乳酸值与 BIOSEN Med L 获得的乳酸值密切相关。首次皮下植入（狗）强调了离体获得的特征：水合 30 分钟后，乳酸导管遵循 BIOSEN Med L 用腿部静脉样本离体测量的乳酸浓度。
An amperometric lactate oxidase catheter has been developed for in vivo application to real-time lactate monitoring. The electrochemical behaviour of the 1 × 3 mm Pt-Ag/AgCl thin film electrode is not significantly influenced by lactate oxidase-polyurethane covering. Gamma-irradiation (25 kGy) is suitable for the sterilization procedure. The final lactate catheter is characterized by a linear concentration range between 0·5 and 20 mmol/l lactate with a sensitivity around 2 nA mmol−11−1lactate. The accuracy is demonstrated by the measurement of control sera. Both physiological and pathological materials correlated well with the declared values. The dry stored lactate catheter needs about 10 min for hydration and is characterized by response times t98%of less than 2 min. Ex vivo whole blood measurements using the lactate catheter (y) give a correlation with the BIOSEN Med L (x) of y = (1·010x + 0·513) mmol/l (r = 0·9748). Lactate values obtained by continuous catheter operation ex vivo correlate well with those obtained by BIOSEN Med L. First subcutaneous implantation (dog) underlines the characteristics obtained ex vivo: after 30 min hydration the lactate catheter follows the lactate concentration measured ex vivo with samples from the leg vein by BIOSEN Med L.