Microbacterium halophytorum sp. nov., a novel endophytic actinobacterium isolated from halophytes
Microbacterium halophytorum sp. nov., a novel endophytic actinobacterium isolated from halophytes
复制标题
盐生微杆菌 sp.
DOI：
10.1099/ijsem.0.003092
复制
发表时间：
2018
影响因子：
2.8
通讯作者：
Li Wen Jun
中科院分区：
文献类型：
--
作者：
Li Yan Ru;Zhu Zhi Nan;Li Yu Qian;Xiao Min;Han Ming Xian;Wadaan Mohammed A M;Hozzein Wael N;An Deng Di;Li Wen Jun
从古尔班通古特沙漠南缘盐生植物中分离到两株放线菌YJYP 303t和YZYP 518。革兰氏染色阳性，需氧，短杆状，无鞭毛。在4-44°C、pH 6.0-12.0和高达15% (w/v) NaCl的存在下，这两种菌株都能生长。基于16S rRNA基因序列的系统发育分析表明，这两株菌株与microbacterium属成员相关。在系统发育树上，两株菌株与耐盐微菌syim 70130T（16S rRNA基因序列同源性为97.58%）和大众微菌likctc 29152T（同源性为96.54%）共享一个分支。菌株YJYP 303t和YZYP 518 toM的平均核苷酸同源性值。测定的耐卤性分别为79.97%和80.03%。菌株YJYP 303和YZYP 518的基因组DNA G+C含量分别为69.72%和70.57%。主要脂肪酸为前iso- c15: 0、前iso- c17: 0和前iso-C16: 0。呼吸醌类以MK-11为主，其次为MK-10和MK-12。肽聚糖的胞酸类型为n -乙二醇基。全细胞糖是甘露糖、核糖、鼠李糖、葡萄糖、半乳糖和两种未知糖。细胞壁氨基酸为谷氨酸、鸟氨酸、甘氨酸和丙氨酸。极性脂质为二磷脂酰甘油、磷脂酰甘油、一种未识别的磷脂和一种未识别的糖脂。根据本研究提供的证据，菌株YJYP 303和YZYP 518被鉴定为microbacterium属的一个新种成员，并将其命名为microbacteriumhalophytorumsp。11月被提议。型应变为YJYP 303T（=CGMCC 1.16264T=KCTC 49100T）。
Two actinobacterial strains, YJYP 303Tand YZYP 518, were isolated from two species of halophytes collected from the southern edge of the Gurbantunggut Desert. Cells were Gram-stain-positive, aerobic, short rods and without flagella. Growth of the two strains was found to occur at 4–44 °C, pH 6.0–12.0 and in the presence of up to 15 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genusMicrobacterium. In the phylogenetic tree, the two strains shared a clade withMicrobacterium halotoleransYIM 70130T(97.58 % 16S rRNA gene sequence identity) andMicrobacterium populiKCTC 29152T(96.54 %). The average nucleotide identity values of strain YJYP 303Tand YZYP 518 toM. halotoleransYIM 70130Twere determined to be 79.97 and 80.03 %, respectively. The genomic DNA G+C contents of strains YJYP 303Tand YZYP 518 were 69.72 and 70.57 %, respectively. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0and iso-C16 : 0. The predominant respiratory quinones was MK-11, followed by MK-10 and MK-12. The muramic acid type of peptidoglycan wasN-glycolyl. The whole-cell sugars were mannose, ribose, rhamnose, glucose, galactose and two unidentified sugars. The cell-wall amino acids were glutamic acid, ornithine, glycine and alanine. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. On the basis of the evidence presented in this study, strains YJYP 303Tand YZYP 518 are characterized as members of a novel species in the genusMicrobacterium, for which the nameMicrobacteriumhalophytorumsp. nov. is proposed. The type strain is YJYP 303T(=CGMCC 1.16264T=KCTC 49100T).